Results 301 to 310 of about 174,288 (316)

Packaging of HCV-RNA into lentiviral vector

Biochemical and Biophysical Research Communications, 2011
The advent of infectious molecular clones of Hepatitis C virus (HCV) has unlocked the understanding of HCV life cycle. However, packaging of the genomic RNA, which is crucial to generate infectious viral particles, remains poorly understood. Molecular interactions of the domain 1 (D1) of HCV Core protein and HCV RNA have been described in vitro.
Vincent, Caval, Eric, Piver, Roland, Ivanyi-Nagy, Jean-Luc, Darlix, Jean-Christophe, Pagès   +4 more
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Packaging of heterologous RNAs by a minimal bovine leukemia virus RNA packaging signal into virus particles

Archives of Virology, 2005
A minimal bovine leukemia virus (BLV) RNA packaging sequence (E) required for heterologous RNAs to be packaged into BLV particles was analyzed. The BLV E was inserted into a non-viral vector, pLacZ, in order to determine if packaging of the non-viral vector RNA would occur. The construct was transfected into cells chronically infected with BLV in order
N A, Jewell, L M, Mansky
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HIV‐1 RNA Packaging

2007
Publisher Summary This chapter focuses on the concept of human immunodeficiency virus type 1 (HIV‐1) RNA packaging. RNA encapsidation by retroviruses is a remarkable process by which the virus negotiates the trafficking of a minority species of mRNA through a particular cellular pathway to become its genome.
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Structure of B77 sarcoma virus RNA: stabilization of RNA after packaging

Journal of Virology, 1975
Extracellular maturation of Bratislava 77(B77) sarcoma virus RNA involves a stabilization of linkage between 35S subunits since the Tm of 60 to 70S RNA in the presence of 0.5 M NaCl increases from 56 to 67.5 C as the age of the virus increases. This stabilization process is strongly temperature dependent; the rate at 45 C is increased fourfold over the
C M, Stoltzfus, P N, Snyder
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Revolution in an RNA-packaging capsid

Science, 2021
Directed Evolution Artificial nucleocapsid proteins, which could be analogous to those used by viruses to package their genomes, are a promising way to protect and deliver RNAs. Using an escalating challenge by nucleases, Tetter et al. evolved a protein that forms multimeric, spherical cages into a highly efficient capsid that selectively packages its ...
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Retroviral RNA packaging: a review

1994
In retroviruses, the "Gag" or core polyprotein is capable of assembling into virus particles and packaging the genomic RNA of the virus. How this protein recognizes viral RNA is not understood. Gag polyproteins contain a zinc-finger domain; mutants with changes in this domain assemble into virions, but a large fraction of these particles lack viral RNA.
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Retroviral RNA Packaging: Sequence Requirements and Implications

1990
Unlike many animal viruses, infection by retroviruses generally does not lead to cessation of host RNA synthesis. Despite the high levels of host RNA in infected cells, the vast majority of retroviral particles contain a precise genomic complex consisting of two molecules of genomic RNA, rather than cellular or subgenomic viral mRNAs.
M L, Linial, A D, Miller
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Genome Packaging by Spherical Plant RNA Viruses

Annual Review of Phytopathology, 2006
Abstract  The majority of positive-strand RNA viruses of plants replicate and selectively encapsidate their progeny genomes into stable virions in cytoplasmic compartments of the cell where the opportunity to copackage cellular RNA also exists. Remarkably, highly purified infectious virions contain almost exclusively viral RNA, suggesting that ...
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RNA Secondary Structure Analysis Using the Vienna RNA Package

Current Protocols in Bioinformatics, 2003
AbstractThis Current Protocols in Bioinformatics unit documents how to use the Vienna RNA package for RNA secondary structure analysis. Possible tasks include structure prediction for single sequences, prediction of consensus structures, and sequence design.
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Nucleic Acid Packaging of RNA Viruses

2006
Viruses are obligate intracellular parasites composed of virus-specific nucleic acid and a protective coat. During virus assembly, the critical stage is the specific recognition of the viral genome with the maturing virus capsid leading to encapsidation (packaging) of the genome to the protective capsid.
Markus Jaakko Pirttimaa, Dennis Henry Bamford   +1 more
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