Parasites & Vectors, 2019 Background Avian haemosporidian parasites can cause severe disease in their hosts due to excessive exo-erythrocytic merogony and anaemia caused by blood stages.
Tanja Himmel +8 more
doaj +1 more source
RNA Probes for Visualization of Sarcin/ricin Loop Depurination without Background Fluorescence. [PDF]
Chem Asian J, 2022 Klimek R +7 more
europepmc +1 more source
FEBS Letters, EarlyView.Obesity raises blood levels of PAI‐1, a protein linked to metabolic dysfunction‐associated steatotic liver disease in people with obesity. In female mice fed a high‐fat diet, partially lowering PAI‐1 led to smaller subcutaneous fat cells and lower liver cholesterol, without changing body weight or insulin sensitivity.
Claudia E. Ramirez Bustamante +10 more
wiley +1 more source
Current techniques for visualizing RNA in cells [version 1; referees: 2 approved]
F1000Research, 2016 Labeling RNA is of utmost interest, particularly in living cells, and thus RNA imaging is an emerging field. There are numerous methods relying on different concepts ranging from hybridization-based probes, over RNA-binding proteins to chemo-enzymatic ...
Lilith V.J.C. Mannack +2 more
doaj +1 more source
Single-probe RNA FISH in Yeast
BIO-PROTOCOL, 2018 Quantitative profiling of mRNA expression is an important part of understanding the state of a cell. The technique of RNA Fluorescence In Situ Hybridization (FISH) involves targeting an RNA transcript with a set of 40 complementary fluorescently labeled DNA oligonucleotide probes.
Gable Wadsworth +2 more
openaire +4 more sources
Parylene Technology for Neural Probes Applications [PDF]
, 2008 Neural probes are important tools in detecting and studying neuron activities. Although people have been working on neural probe development for a long time, the current neural probes (including metal-wire probes and silicon neural probes) are still far ...
Pang, Changlin
core +1 more source
Ligand‐dependent transcriptional heterogeneity in cell cycle gene expression delays G1/S entry
FEBS Letters, EarlyView.EGF and HRG induce distinct G1/S progression programs in ErbB2‐amplified BT474 breast cancer cells. Despite activating the potent ErbB2–ErbB3 heterodimer, HRG does not accelerate cell‐cycle entry. Instead, EGF promotes earlier restriction‐point passage via ERK–FOS signaling, whereas HRG activates the AKT–MYC axis, driving transcriptional heterogeneity ...
Ririn Rahmala Febri +5 more
wiley +1 more source
Ethylenediamine derivatives efficiently react with oxidized RNA 3' ends providing access to mono and dually labelled RNA probes for enzymatic assays and in vivo translation. [PDF]
Nucleic Acids Res, 2022 Mamot A +5 more
europepmc +1 more source
Functional characterization of Ysh1p, the yeast endonuclease involved in 3" end processing and in transcription termination of RNA polymerase II transcripts [PDF]
, 2008 Eukaryotic RNA polymerase II (RNAP II) is involved in the synthesis of two major classes of transcripts: messenger RNAs (mRNAs) and small nuclear and small nucleolar RNAs.
Garas, Monika
core +1 more source
Synergistic perspectives—How single‐molecule biophysics complement biochemical understanding
FEBS Letters, EarlyView.In this review, we discuss how ensemble biochemistry and single‐molecule approaches are complementary, outline commonly used single‐molecule techniques, and illustrate their relevance through two representative case studies: chromatin organization by SMC complexes and pathway choice during DNA double‐strand break repair.
Sara De Bragança +2 more
wiley +1 more source