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Cotranscriptional RNA Chemical Probing

2022
Cotranscriptional folding is a fundamental step in RNA biogenesis and the basis for many RNA-mediated gene regulation systems. Understanding how RNA folds as it is synthesized requires experimental methods that can systematically identify intermediate RNA structures that form during transcription.
Courtney E, Szyjka, Eric J, Strobel
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RNA imaging by chemical probes

Advanced Drug Delivery Reviews, 2019
Sequence-specific detection of intracellular RNA is one of the most important approaches to understand life phenomena. However, it is difficult to detect RNA in living cells because of its variety and scarcity. In the last three decades, several chemical probes have been developed for RNA detection in living cells.
Fumiaki, Tomoike, Hiroshi, Abe
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Structural Probing of RNA Thermosensors

2009
Chemical probing of RNA structure has become one of the most popular approaches to map the conformation of RNA molecules of various sizes under well-defined experimental conditions. The method monitors the sensitivity of each nucleotide to various chemicals, which reflects its hydrogen-bonding environment within the RNA molecule.
Chiaruttini, C.   +2 more
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RNA stable-isotope probing

Nature Protocols, 2007
At the heart of microbial ecology lies a true scientific dichotomy. On the one hand, we know microbes are responsible for processes on which all other life on Earth is dependent; their removal would mean the cessation of all known life. However, in opposition, the majority of extant microbial species in natural environments have never been cultured or ...
Andrew S, Whiteley   +3 more
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Probing the RNA–protein interface

Nature Reviews Genetics, 2020
Corley et al. report fSHAPE, a simplified strategy for in vivo transcriptome-wide footprinting with SHAPE structure-probing techniques.
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