Results 141 to 150 of about 439,420 (307)
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
FEBS Open Bio, EarlyView.Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
Robust Estimators are Hard to Compute [PDF]
In modern statistics, the robust estimation of parameters of a regression hyperplane is a central problem. Robustness means that the estimation is not or only slightly affected by outliers in the data. In this paper, it is shown that the following robust
Bernholt, Thorsten
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Robust estimation of the Hurst parameter and selection of an onset scaling. [PDF]
, 2009 We consider the problem of estimating the Hurst parameter for long-range dependent processes using wavelets. Wavelet techniques have been shown to effectively exploit the asymptotic linear relationship that forms the basis of constructing an estimator ...
Park, Cheolwoo, Park, Juhyun
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FEBS Open Bio, EarlyView.Despite frogs avoiding low temperatures, examination of four salamander species revealed that none avoided cold and all possessed cold tolerance. Functional analysis of TRPM8, a cold sensor, showed that all salamander TRPM8s had lost their cold sensitivity.
Tadahiro Sawao +3 more
wiley +1 more source
A NEW METHOD OF ROBUST LINEAR REGRESSION ANALYSIS: SOME MONTE CARLO EXPERIMENTS [PDF]
This paper has elaborated upon the deleterious effects of outliers and corruption of dataset on estimation of linear regression coefficients by the Ordinary Least Squares method.
Sudhanshu Kumar MISHRA
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Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system
FEBS Open Bio, EarlyView.An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková +3 more
wiley +1 more source
, 2001 Copyright [2001] IEEE. This material is posted here with permission of the IEEE. Such permission of the IEEE does not in any way imply IEEE endorsement of any of Brunel University's products or services.
Wang, Z, Burnham, KJ
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Systemic dysregulation of apolipoproteins in amyotrophic lateral sclerosis serum
FEBS Open Bio, EarlyView.Amyotrophic lateral sclerosis (ALS) is a fatal disease that damages motor neurons. This study found that people with ALS show significant changes in blood fats and the proteins that carry them. Several apolipoproteins were higher, lipid balances were altered, and normal protein–lipid relationships were disrupted.
Finula I. Isik +6 more
wiley +1 more source
Robust estimation in linear regression models with fixed effects [PDF]
In this work we extend the procedure proposed by Peña and Yohai (1999) for computing robust regression estimates in linear models with fixed effects. We propose to calculate the principal sensitivity components associated to each cluster and delete the ...
Betsabe Perez +2 more
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Worst-case estimation and asymptotic theory for models with unobservables [PDF]
, 2004 This paper proposes a worst-case approach for estimating econometric models containing unobservable variables. Worst-case estimators are robust against the adverse effects of unobservables.
Vidal-Sanz, Jose M. +1 more
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