Results 101 to 110 of about 232,265 (304)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Comparison and Joint Evaluation of Importance, Redundancy and Robustness Indicators Applied to Aging Prestressed Concrete Bridges

Nordic Concrete Research
A bridge failure can result in significant social, economic, and environmental problems; therefore, its reliability and risk management are essential.
Sarmiento Silvia, Thöns Sebastian, González-Libreros Jaime, Björnsson Ivar, Sas Gabriel   +4 more
doaj   +1 more source

Robust collaborative mesh networking with large-scale distributed wireless heterogeneous terminals in industrial cyber-physical systems

International Journal of Distributed Sensor Networks, 2017
Industrial cyber-physical system is defined as transformative technologies for upgrading the traditional industrial mode. Wireless mesh network becomes a typical technology in industrial cyber-physical system for the network communication with large ...
Tao Wang, Jun Liu, Lianglun Cheng, Hong Xiao   +3 more
doaj   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

From risk to resilience: Quantifying water security through reliability and robustness analysis with simulation–optimization framework

Journal of Hydrology: Regional Studies
Study Region: Taiwan’s six major municipalities face pronounced wet–dry seasonal contrasts and rely heavily on monsoon-driven inflows to sustain year-round supply.
Chia-Wen Wu, Frederick N.-F. Chou, Dawn Brady   +2 more
doaj   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Bayes reliability measures of Lognormal and inverse Gaussian distributions under ML-II ε-contaminated class of prior distributions [PDF]

In this paper we employ ML-II ε-contaminated class of priors to study the sensitivity of Bayes Reliability measures for an Inverse Gaussian (IG) distribution and Lognormal (LN) distribution to misspecification in the prior.
Sinha, Pankaj, Jayaraman, Prabha
core   +1 more source

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

Systemic dysregulation of apolipoproteins in amyotrophic lateral sclerosis serum

FEBS Open Bio, EarlyView.
Amyotrophic lateral sclerosis (ALS) is a fatal disease that damages motor neurons. This study found that people with ALS show significant changes in blood fats and the proteins that carry them. Several apolipoproteins were higher, lipid balances were altered, and normal protein–lipid relationships were disrupted.
Finula I. Isik, Russell Pickford, Hannah C. Timmins, Olivier Piguet, Glenda M. Halliday, Matthew C. Kiernan, Woojin Scott Kim   +6 more
wiley   +1 more source

Validation of Replicable Pipeline 3D Surface Reconstruction for Patient-Specific Abdominal Aortic Lumen Diagnostics

BioMed
Background: Accurate prognoses are challenging in high-risk vascular conditions, such as abdominal aortic aneurysms, and limited diagnostic standards, decision-making criteria, and data semantics often hinder clinical reliability and impede diagnostics ...
Edoardo Ugolini, Giorgio La Civita, Moad Al Aidroos, Samuele Salti, Giuseppe Lisanti, Emanuele Ghedini, Gianluca Faggioli, Mauro Gargiulo, Giovanni Rossi   +8 more
doaj   +1 more source