Results 121 to 130 of about 638,105 (305)
The Multi-Parent Advanced Generation Inter-Cross (MAGIC) population is a powerful tool for dissecting the genetic architecture controlling natural variation in complex traits.
Brenda Anabel López-Ruiz +5 more
doaj +1 more source
In this study, we developed a deep learning method for mitotic figure counting in H&E‐stained whole‐slide images and evaluated its prognostic impact in 13 external validation cohorts from seven different cancer types. Patients with more mitotic figures per mm2 had significantly worse patient outcome in all the studied cancer types except colorectal ...
Joakim Kalsnes +32 more
wiley +1 more source
Research on the Physiological Mechanisms of Nitrogen in Alleviating Plant Drought Tolerance
Drought represents a paramount constraint on global agricultural productivity, imposing severe limitations on crop yield and quality across diverse agroecosystems.
Xichao Sun +4 more
doaj +1 more source
Deciphering Root Architectural Traits Involved to Cope With Water Deficit in Oat
Drought tolerance is a complex phenomenon comprising many physiological, biochemical and morphological changes at both aerial and below ground levels. We aim to reveal changes on root morphology that promote drought tolerance in oat in both seedling and ...
Francisco J. Canales +4 more
doaj +1 more source
Untapping root system architecture for crop improvement [PDF]
Drought leads to greater yield loss in crops than any other abiotic stress. Roots form a plant’s first line of defense against water deficit, and in this issue Gao and Lynch (pages 4533–4546) report that fewer but longer crown roots facilitate water acquisition from subsoil and thus improve drought tolerance in maize.
openaire +2 more sources
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
MRI in soils: determination of water concent changes due to root water uptake by means of a multi-slice-multi-echo sequence (MSME) [PDF]
Root water uptake by ricinus communis (castor bean) in fine sand was investigated using MRI with multiecho sampling. Before starting the experiments the plants germinated and grew for 3 weeks in a cylindrical container with a diameter of 9 cm ...
As, H., van +5 more
core +2 more sources
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
The use of bioinoculants aligns with ecological intensification in agriculture, but their effects on crop performance and soil microbiota under different fertilization regimes remain unclear.
Raquel Gomes de Oliveira +8 more
doaj +1 more source
Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke +4 more
wiley +1 more source

