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Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

open access: yesFEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee   +3 more
wiley   +1 more source
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Root Cause Analysis

JAMA, 2021
In the summer of 2020, a patient at a major Veteran Affairs medical center in the western United States experienced an unwitnessed fall in their telemetry patient ward. This incident resulted in the patient receiving a fracture in his/her C5 cervical spine area. This eventually led to his/her death the following day.
  +6 more sources

Metadata For Root Cause Analysis

ECMS 2021 Proceedings edited by Khalid Al-Begain, Mauro Iacono, Lelio Campanile, Andrzej Bargiela, 2021
The paper is devoted to the task of finding the root cause of anomaly in a distributed information and computing system. An approximate approach is considered to detect implicit anomalies with accuracy to the object (of a component of the technical device, a node of a network infrastructure, an application or of an information resource).
Alexander A. Grusho   +4 more
openaire   +1 more source

Root-Cause Analysis

EDFA Technical Articles, 2020
Abstract A root-cause analysis is used to identify the initiating, or root, of a causal chain that leads to an observed undesirable outcome. It is useful in helping managers to focus their problem-solving efforts on providing remedies to issues that actually prevent the undesirable outcome from recurring.
Greg Fisher   +2 more
openaire   +2 more sources

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