Results 121 to 130 of about 3,391,638 (305)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Evaluation of drought-tolerant chickpea genotypes (Cicer arietinum L.) using morphophysiological and phytochemical traits

Frontiers in Plant Science
Chickpea (Cicer arietinum L.), through a series of morphological, physiological, biochemical, and molecular changes, would tolerate abiotic stresses such as water deficiency.
Bahman Fazeli-Nasab, Saeedreza Vessal, Abdolreza Bagheri, Saeid Malekzadeh-Shafaroudi   +3 more
doaj   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Variation in Root Traits and Root-Endophyte Interactions in Primary Synthetic Wheat Derived from Aegilops tauschii Collected from Diverse Soil Types

Agronomy
Modern wheat breeding has largely emphasized aboveground traits, often at the expense of belowground characteristics such as root biomass, architecture, and beneficial microbial associations.
Ahmed Khaled Hassan Mohammedali, Nasrein Mohamed Kamal, Yasir Serag Alnor Gorafi, Izzat Sidahmed Ali Tahir, Hisashi Tsujimoto, Takeshi Taniguchi   +5 more
doaj   +1 more source

REVISITING HYSTERESIS IN UNEMPLOYMENT FOR TEN EUROPEAN COUNTRIES: AN EMPIRICAL NOTE ON A MORE POWERFUL NONLINEAR (LOGISTIC) UNIT ROOT [PDF]

In this empirical note we use a more powerful nonlinear (logistic) unit root test advanced by Leybourne et al. (1998) to investigate the hysteresis in unemployment for ten European countries for the period 1961-2003.
Yuan-Hong Ho, Chung-Ju Huang, Tsangyao Chang   +2 more
core  

Avidin is evolutionarily conserved in fish but dispensable for development and resistance against Streptococcus agalactiae in zebrafish

FEBS Open Bio, EarlyView.
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti, Markus J. T. Ojanen, Mataleena Parikka, Otto Kauko, Mika Rämet, Vesa P. Hytönen   +5 more
wiley   +1 more source

Deciphering the genetic landscape of seedling drought stress tolerance in wheat (Triticum aestivum L.) through genome-wide association studies

Frontiers in Plant Science
Wheat is an important cereal crop constrained by several biotic and abiotic stresses including drought stress. Understating the effect of drought stress and the genetic basis of stress tolerance is important to develop drought resilient, high-yielding ...
Santosh Gudi, Priyanka Halladakeri, Gurjeet Singh, Gurjeet Singh, Pradeep Kumar, Pradeep Kumar, Satinder Singh, Khairiah Mubarak Alwutayd, Diaa Abd El-Moneim, Achla Sharma   +9 more
doaj   +1 more source

Bill Clinton Gubernatorial record group; Education series; Paul Root subseries

, 1979
This collection contains working papers of Paul Root, a special advisor to Governor Bill Clinton on education, aging, and religion from January 1983 to August ...
Root, Paul
core   +1 more source

Structural and biochemical insights into the thermostable esterase Ta0887 from Thermoplasma acidophilum

FEBS Open Bio, EarlyView.
In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey, Miriam Livier Llamas‐García, Gabriela M. Montero‐Morán, Leticia Santos, Samuel Lara‐González   +4 more
wiley   +1 more source

Salmonella enterica serovar typhi limits the potency of typhoid toxin and ADP‐ribosylating toxin AB to establish a persistent infection

FEBS Open Bio, EarlyView.
The two catalytic subunits of typhoid toxin dissociate from the holotoxin in the ER of an intoxicated cell, but only CdtB exits the ER to generate immunosuppressive effects. PltA is retained in the ER and sequestered from its cytosolic target, thus allowing the anti‐inflammatory effects of CdtB to promote intestinal colonization.
Maria C. Zabala‐Rodriguez, Juliette K. Tinker, Teresa Frisan, Taj Azarian, Ken Teter   +4 more
wiley   +1 more source