Results 111 to 120 of about 4,024,921 (250)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source

Braking Force Control for Direct-Drive Brake Units Based on Data-Driven Adaptive Control

Machines
To address the increasing demands for faster response and higher control accuracy in the braking systems of electric and intelligent vehicles, a novel brake-by-wire actuation unit and its braking force control methods are proposed.
Chunrong He, Xiaoxiang Gong, Haitao He, Huaiyue Zhang, Yu Liu, Haiquan Ye, Chunxi Chen   +6 more
doaj   +1 more source

Rotary Luncheon

, 2012
Mailer for the first 'Toledo Rotary Luncheon' with hand written date of 1912.
Toledo Rotary Club
core  

Aquaporin‐3 and aquaporin‐5 impact the development of pancreatic ductal adenocarcinoma spheroids

FEBS Open Bio, EarlyView.
Schematic representation of the role of aquaporin‐3 (AQP3) and aquaporin‐5 (AQP5) in pancreatic ductal adenocarcinoma (PDAC). Both proteins are upregulated in PDAC and are associated with tumor progression and metastatic potential. Silencing AQP3 or AQP5 in PDAC spheroids results in decreased diameter, area, and overall growth, underscoring their key ...
Catarina Pimpão, Diogo M. Engrácia, Graça Soveral, Filipa Mendes   +3 more
wiley   +1 more source

Rotary History

, 2012
A typed timeline of the local Rotary clubs and their years of ...
Toledo Rotary Club
core  

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

From energy provision to protein synthesis: Tunnelling nanotubes as mediators of intercellular metabolic cooperation in cancer

FEBS Open Bio, EarlyView.
The cytoskeleton‐mediated transport of mitochondria via tunnelling nanotubes restores respiration, increases ATP production, rescues cells from apoptosis, activates the AKT/mTOR signalling pathway, promotes cell migration and invasiveness, contributes to cancer progression and treatment resistance.
Stanislava Martínková, Jan Trnka
wiley   +1 more source

Rotary Emblem

, 2012
This photo shows the progression of the Rotary Symbol from 1906 ...
Toledo Rotary Club
core  

Modelagem de motor de indução linear utilizando o metodo de elementos finitos em 2D [PDF]

, 2001
Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro Tecnológico.O presente trabalho aborda o desenvolvimento e a implementação de um método de análise de dispositivos eletromagnéticos lineares alimentados por circuitos elétricos ...
Deás Yero, Douglas Rebot
core