Results 61 to 70 of about 5,871,393 (260)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

open access: yesFEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen   +5 more
wiley   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

open access: yesFEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh   +2 more
wiley   +1 more source

Theoretical Analysis of Different Classifiers under Reduction Rough Data Set: A Brief Proposal

open access: yes, 2023
Rough set plays vital role to overcome the complexities, vagueness, uncertainty, imprecision, and incomplete data during features analysis. Classification is tested on certain dataset that maintain an exact class and review process where key attributes ...
Kamal, S, Dey, N, Hossain, S, Ripon, SH
core   +1 more source

From patient advocacy to patient‐driven research: Building active partnerships beginning at the bench to reach the bedside

open access: yesFEBS Open Bio, EarlyView.
Research is strongest when conducted alongside patients, not just about them. Patient research organizations help integrate patient perspectives into research priorities, study design, and scientific meetings, leading to meaningful patient outcomes and development of relevant therapies.
Jenica H. Kakadia   +9 more
wiley   +1 more source

Rough Forgetting

open access: yes, 2020
Recent work in the area of Knowledge Representation and Reasoning has focused on modification and optimization of knowledge bases (KB) through the use of forgetting operators of the form forget(KB, (R) over bar), where (R) over bar is a set of relations ...
Andrzej Szałas   +5 more
core   +1 more source

GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma

open access: yesFEBS Open Bio, EarlyView.
GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain   +11 more
wiley   +1 more source

New Uncertainty Measure of Rough Fuzzy Sets and Entropy Weight Method for Fuzzy-Target Decision-Making Tables

open access: yesJournal of Applied Mathematics, 2014
In the rough fuzzy set theory, the rough degree is used to characterize the uncertainty of a fuzzy set, and the rough entropy of a knowledge is used to depict the roughness of a rough classification.
Huani Qin, Darong Luo
doaj   +1 more source

Rough set methodology in meta-analysis - a comparative and exploratory analysis [PDF]

open access: yes
We study the applicability of the pattern recognition methodology "rough set data analysis" (RSDA) in the field of meta analysis. We give a summary of the mathematical and statistical background and then proceed to an application of the theory to a meta ...
Rupp, Thomas
core  

BCG vaccination potentiates oxidative phosphorylation in neonatal myeloid‐derived suppressor cells

open access: yesFEBS Open Bio, EarlyView.
BCG vaccination enhances oxidative phosphorylation in neonatal MDSCs, impairing their immunosuppressive function. It upregulates electron transport chain genes and mitochondrial activity, increasing ATP and oxygen consumption. Pharmacological OXPHOS inhibition partially restores suppressive capacity, confirming causality.
Yingying Chen, Hui Li
wiley   +1 more source

Rough I-Convergence

open access: yesDemonstratio Mathematica, 2014
In this work, using the concept of I-convergence and using the concept of rough convergence, we introduced the notion of rough I-convergence and the set of rough I-limit points of a sequence and obtained two rough I-convergence criteria associated with ...
Dündar Erdinç, Çakan Celal
doaj   +1 more source

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