Results 31 to 40 of about 25,345 (219)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

open access: yesFEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point   +7 more
wiley   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

open access: yesFEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill   +4 more
wiley   +1 more source

Tribological Investigation of Plasma-Based Coatings for Use in Quasi-Monolithic Engine Cylinder Bores

open access: yesMetals
This study evaluates the tribological characteristics of quasi-monolithic engine cylinder coatings and piston rings using a custom-built linear reciprocating tribometer.
Siddharth Banerjee   +3 more
doaj   +1 more source

An Axiomatic Approach to the Roughness Measure of Rough Sets [PDF]

open access: yesFundamenta Informaticae, 2011
In Pawlak's rough set theory, a set is approximated by a pair of lower and upper approximations. To measure numerically the roughness of an approximation, Pawlak introduced a quantitative measure of roughness by using the ratio of the cardinalities of the lower and upper approximations.
openaire   +2 more sources

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

open access: yesFEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen   +9 more
wiley   +1 more source

Effect of the Artificial Bed Roughness’s Shape on Hydraulic Jump’s Characteristics [PDF]

open access: yesعلوم و مهندسی آبیاری, 2018
Hydraulic jumps are the most important type of rapidly variable flow that occur in from the supercritical state to the subcritical state with the sudden increase of the water free surface in a relatively short path, and usually has a high kinetic energy ...
Saeid Salamiasl   +2 more
doaj   +1 more source

Refinement of amino‐acid conformation vs. difference density maps in time‐resolved serial femtosecond crystallography data analysis

open access: yesFEBS Open Bio, EarlyView.
The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong   +3 more
wiley   +1 more source

Nicotine-induced changes in surface properties of restorative materials and dental enamel: An in vitro study on flavored e-cigarette exposure

open access: yesTobacco Induced Diseases
Introduction This study aimed to assess the effects of various nicotine concentrations in flavored electronic cigarettes (e-cigarettes) on the surface roughness, microhardness, and color stability of restorative materials and enamel structure.
Maria Salem Ibrahim   +5 more
doaj   +1 more source

Long‐Term Follow‐Up of Chemotherapy‐Associated Biological Aging in Women With Early Breast Cancer

open access: yesAging and Cancer, EarlyView.
Women threated with adjuvant chemotherapy for early breast cancer have sustained long‐term increase in p16INK4a,, a robust marker of cell senescence, suggesting a chemotherapy‐associated age acceleration. p16INK4a as well as other biomarkers may identify patients at greatest risk for senescence‐related diseases of aging.
Hyman B. Muss   +12 more
wiley   +1 more source

A simplified method on estimation of forest roughness by use of aerial LIDAR data

open access: yesEnergy Science & Engineering, 2019
In the last decade, satellite‐based measurements combined with local land cover information have produced datasets with a very detailed land cover description.
Ferhat Bingöl
doaj   +1 more source

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