Results 311 to 320 of about 1,081,218 (337)

Establishment of Singleplex and Duplex TaqMan RT-qPCR Detection Systems for Strawberry Mottle Virus (SMoV) and Strawberry Vein Banding Virus (SVBV). [PDF]

Plants (Basel)
Xu T, Gao D, Wu M, Wang H, He C.
europepmc   +1 more source

Development of a Quadruplex RT-qPCR Assay for Rapid Detection and Differentiation of PRRSV-2 and Its Predominant Genetic Sublineages in China. [PDF]

Viruses
Ye G, Xiong S, Su Z, Chen G, Liu S, Wang Z, Chen H, Zhang A.   +7 more
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A sensitive multiplex RT-qPCR assay to detect SARS-CoV-2 in respiratory samples

Halliday A, Morales-Aza B, Duale K, Lait P, Bristol Vaccine Centre Microbiology & Molecular Laboratories, Milligan R, Hutchings S, Pymont H, Vipond B, Muir P, Waterfall C, Coghill J, Carr J, Plested E, Oliver J, Fidler K, Relton C, Snape MD, Davidson AD, Helps C, Finn A.   +20 more
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Detection of Viroids Using RT-qPCR

2021
Viroids are the smallest known infectious pathogens. They are nonprotein-encoding, single-stranded, circular, naked RNA molecules that can cause several diseases in economically important crops. With the advent of thermal cyclers incorporating fluorescent detection, reverse transcription coupled to the quantitative polymerase chain reaction (RT-qPCR ...
Subhas, Hajeri, Georgios, Vidalakis, Raymond K, Yokomi   +2 more
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Novel duplex RT‐qPCR for animal rabies surveillance

Transboundary and Emerging Diseases, 2022
Rabies is a lethal zoonosis affecting mammals worldwide. Diagnosis of rabies follows international standard protocols, primarily relying on direct immunofluorescence (DI) followed by mouse inoculation test (MIT). WHO recommends molecular biology techniques such as RT-qPCR for replacing MIT to diagnose rabies in animal samples. Recently, a real-time PCR
Guilherme Augusto Minozzo, Thaila Francini Corona, Elaine Cristina Rodrigues da Cruz, Wagner Antonio Chiba de Castro, Louise Bach Kmetiuk, Andrea Pires dos Santos, Alexander Welker Biondo, Irina Nastassja Riediger   +7 more
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Qualitätsmanagement in der RT-qPCR

BIOspektrum, 2012
Several aspects in the numerous steps of a reverse transcription (RT) quantitative PCR may interfere with the result’s validity. Therefore, before starting the proper investigation, a particular assay establishment is required.
Catrin Wernicke, Philipp Franke, Lars Radke, Stephan Berge, Marcus Frohme   +4 more
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Whole-Genome RT-qPCR MicroRNA Expression Profiling

2011
MicroRNAs (miRNAs) are small noncoding RNA molecules that function as negative regulators of gene expression. They are essential components of virtually every biological process and deregulated miRNA expression has been reported in a multitude of human diseases including cancer.
Pieter, Mestdagh, Stefaan, Derveaux, Jo, Vandesompele   +2 more
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Multigene Combined Detection by RT-qPCR Using Cytological Specimens

Acta Cytologica, 2021
<b><i>Objective:</i></b> The aim of the study was to investigate the mutation status of multiple driver genes by RT-qPCR and their significance in advanced lung adenocarcinoma using cytological specimens. <b><i>Materials and Methods:</i></b> 155 cytological specimens that had been diagnosed with lung ...
Yang, Ma, Jingxia, Zhao, Yun, Du, Rui, Wang, Xiaokun, Ji, Juan, Wu, Xiao, Guo, Ying, Liu, Yan, Zhang   +8 more
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Quantitation of MicroRNAs by Real-Time RT-qPCR

2010
MicroRNAs (miRNAs) are ∼22 nucleotide regulatory RNA molecules that play important roles in controlling developmental and physiological processes in animals and plants. Measuring the level of miRNA expression is a critical step in methods that study the regulation of biological functions and that use miRNA profiles as diagnostic markers for cancer and ...
Caifu, Chen, Ruoying, Tan, Linda, Wong, Richard, Fekete, Jason, Halsey   +4 more
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Stem-Loop RT-qPCR for MicroRNA Expression Profiling

2011
Quantification of the microRNAs (miRNAs) in cells or tissues is a crucial step in understanding their biological functions. Development of the stem-loop reverse transcription procedure and TaqMan(®) miRNA assays enables accurate detection of miRNA expression levels by quantitative PCR.
James, Hurley, Doug, Roberts, Andrew, Bond, David, Keys, Caifu, Chen   +4 more
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