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Gene Expression Analysis in Bacteria by RT-qPCR
2019Reverse-transcription quantitative real-time polymerase chain reaction (RT-qPCR) using fluorescent DNA-binding dyes is now a gold-standard methodology to study bacterial gene expression through relative quantitation of target mRNAs under specific experimental conditions, and recent developments in the technology allow for gene expression analysis in ...
Danilo J P G, Rocha +3 more
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RT-qPCR work-flow for single-cell data analysis
Methods, 2013Individual cells represent the basic unit in tissues and organisms and are in many aspects unique in their properties. The introduction of new and sensitive techniques to study single-cells opens up new avenues to understand fundamental biological processes.
Anders, Ståhlberg +4 more
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Analisi dei miRNA mediante RT-qPCR
2018Protocollo di analisi dei miRNA mediante RT-qPCR adottato da CNR-IFC (in collaborazione con CNR-IRPPS e Università del Salento) nell'ambito del progetto GILEAD (Fellowship 2015) "Correlazione dell'andamento dei livelli dei linfociti T CD4+ e dell'HIV-RNA in pazienti naïve e relazione con una differente espressione di miRNA, nell'individuazione ...
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