Results 41 to 50 of about 2,030,676 (301)
Ligand Binding: Molecular Mechanics Calculation of the Streptavidin-Biotin Rupture Force [PDF]
Science, 1996 The force required to rupture the streptavidin-biotin complex was calculated here by computer simulations. The computed force agrees well with that obtained by recent single molecule atomic force microscope experiments. These simulations suggest a detailed multiple-pathway rupture mechanism involving five major unbinding steps.
Grubmüller, H. +2 more
openaire +4 more sources
Degradation mechanism of the von Willebrand factor A2 domain by nattokinase
FEBS Letters, EarlyView.Nattokinase, a natto‐derived protease, exhibits potent antithrombotic effects. This study demonstrates that nattokinase directly cleaves the von Willebrand factor (vWF) A2 domain in vitro. Unlike the native regulator ADAMTS13, nattokinase degrades folded vWF independently of shear stress.
Ryuichi Hyakumoto +3 more
wiley +1 more source
Molecules, 2015 Binding affinity prediction of protein–ligand complexes has attracted widespread interest. In this study, a self-adaptive steered molecular dynamics (SMD) method is proposed to reveal the binding affinity of protein–ligand complexes.
Junfeng Gu, Hongxia Li, Xicheng Wang
doaj +1 more source
On the Interplay Between Force, Temperature, and Electric Fields in the Rupture Process of Mechanophores [PDF]
The use of oriented external electric fields (OEEFs) shows promise as an alternative method for catalyzing chemical reactions. The ability to target a specific bond by aligning it with a bond-weakening electric field may be beneficial in mechanochemical ...
Tarek, Scheele, Tim, Neudecker
core +1 more source
FEBS Letters, EarlyView.We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace +6 more
wiley +1 more source
Experimental validation for the Achilles tendon reinforced with new techniques
Гений oртопедии, 2022 An Achilles tendon injury is most common among subcutaneous tendon ruptures and accounts for 47 %. The purpose was to experimentally evaluate the effectiveness of new methods of the Achilles tendon reinforcement.
Gennadii P. Kotelnikov +4 more
doaj +1 more source
Membranes, 2023 Transforming growth factor β1 (TGF-β1) is critical to cell differentiation, proliferation, and apoptosis. It is important to understand the binding affinity between TGF-β1 and its receptors. In this study, their binding force was measured using an atomic
Gounhanul Shin +3 more
doaj +1 more source
Synergistic perspectives—How single‐molecule biophysics complement biochemical understanding
FEBS Letters, EarlyView.In this review, we discuss how ensemble biochemistry and single‐molecule approaches are complementary, outline commonly used single‐molecule techniques, and illustrate their relevance through two representative case studies: chromatin organization by SMC complexes and pathway choice during DNA double‐strand break repair.
Sara De Bragança +2 more
wiley +1 more source
Using Oriented External Electric Fields to Manipulate Rupture Forces of Mechanophores
Physical Chemistry Chemical Physics, 2023 Oriented External Electric Fields (OEEFs) can catalyze chemical reactions by selectively weakening bonds. This makes them a topic of interest in mechanochemistry, where mechanical force is used to rupture specific bonds in molecules. Using electronic structure calculations based on density functional theory (DFT), we investigate the effect of OEEFs on ...
Tarek Scheele, Tim Neudecker
openaire +2 more sources
Leucine‐rich glioma inactivated 1 (LGI1) is a ganglioside‐binding protein
FEBS Letters, EarlyView.Neuronal hyperexcitability associated with a decrease/absence of the extracellular protein LGI1 has been suggested to be primarily due to the downregulation of Kv1 channel expression. The molecular mechanisms underlying this decrease have not yet been elucidated.
Kévin Debreux +7 more
wiley +1 more source