Results 51 to 60 of about 192,730,823 (302)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

The effect of sedentary behavior during pregnancy on premature rupture of membrane in women above 35 years old

SAGE Open Medicine
Background: Sedentary life is now considered among the main health risks globally among general population and pregnant women. Premature rupture of membranes is a serious pregnancy condition that is a main cause of newborn morbidity and death globally ...
Rehab Hanafy Mahmoud Abdelsamiea, Ghada Mahmoud Khafagy, Hassan Omar Ghareib, Mai Diaa Sarhan   +3 more
doaj   +1 more source

Guiding AlphaFold to predict how Munc13‐1 opens Syntaxin‐1

FEBS Open Bio, EarlyView.
The syntaxin‐1 Habc‐domain (orange), linker (pink) and SNARE motif (yellow) form a closed conformation that binds to Munc18‐1 (violet) and is opened by the Munc13‐1 MUN domain (cyan) to form the SNARE complex that triggers neurotransmitter release.
Madhurima Chattopadhyay, Junjie Xu, Josep Rizo   +2 more
wiley   +1 more source

Exploring the influence of lactoferrin on wound healing and related signaling pathways: a systematic review

FEBS Open Bio, EarlyView.
Lactoferrin promotes wound healing by activating MAPK, PI3K/Akt, and extracellular matrix pathways, enhancing fibroblast remodeling, proliferation, and re‐epithelialization. These mechanisms highlight its therapeutic potential in inflammation control, tissue repair, and remodeling.
Morgana Lüdtke Azevedo, Kátia Cristiane Hall, Matheus dos Santos Fernandez, Geovanna Peter Corrêa, Rafael Guerra Lund   +4 more
wiley   +1 more source

The impact of postoperative pregnancy intervals (t) on preterm delivery and premature rupture of membranes.

, 2022
The impact of postoperative pregnancy intervals (t) on preterm delivery and premature rupture of membranes.
Yue Gao (263086), Yunyun Xiao (2111017), Huali Wang (289309)   +2 more
core   +1 more source

Analysis of the microbiome in maternal, intrauterine and fetal environments based on 16S rRNA genes following different durations of membrane rupture

Scientific Reports, 2023
The incidence of chorioamnionitis and neonatal sepsis increases with the increasing time of rupture of membranes. Changes in the amount and categories of microbiomes in maternal and fetal environments after membrane rupture have yet to be discussed.
Huifen Yin, Jiao Yu, Wei Wu, Xiaotian Li, Rong Hu   +4 more
doaj   +1 more source

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

FEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty, Kelsi R. Hall, Zachary D. Tillett, Liam S. Turk, James S. Davies, Michael J. Currie, Hamish G. Brown, Sepideh Valimehr, Peter D. Mace, Andrew E. Whitten, Santosh Panjikar, Eric Hanssen, Rachel A. North, Renwick C.J. Dobson   +13 more
wiley   +1 more source

Interregional variation in artificial rupture of membranes in midwife-led care at onset of labour.

, 2020
Interregional variation in artificial rupture of membranes in midwife-led care at onset of labour.
Marianne Nieuwenhuijze (5106791), Ank de Jonge (585261), Corine J. Verhoeven (4745109), François G. Schellevis (5650183), Dirkje C. Zondag (8537964), Thomas van den Akker (218592), Anna E. Seijmonsbergen-Schermers (8537961), Caroline C. Geerts (4281835)   +7 more
core   +1 more source

The impact of cone floor transverse diameters on preterm delivery and premature rupture of membranes.

, 2022
The impact of cone floor transverse diameters on preterm delivery and premature rupture of membranes.
Yue Gao (263086), Yunyun Xiao (2111017), Huali Wang (289309)   +2 more
core   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source