MOLECULAR IDENTIFICATION OF S-GENOTYPES OF ALMOND (Prunus dulcis)
Acta Horticulturae, 2001Self-incompatibility (SI) is a mechanism whereby pistils reject pollen carrying the same S-allele. SI promotes out-crossing whilst at the same time, causing problems in crop and fruit production. The knowledge of the S-allele composition, together with that of flowering periods of these cultivars, makes it possible to predict their cross-compatibility ...
null Ma, M.M. Oliveira
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Improved S-genotyping and new incompatibility groups in Japanese plum
Euphytica, 2012The selection of cross-compatible cultivars is essential to ensure fruit set in self-incompatible species like Japanese plum and thus the S-genotype must be determined in order to establish incompatibility groups. In this study an improved Japanese plum S-genotyping method, based in polymerase chain reaction and capillary electrophoresis detection of ...
María E. Guerra +2 more
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Identification of self-incompatibility (S-) genotypes of Chinese apricot cultivars
Euphytica, 2007The S-genotypes of 16 apricot (Prunus armeniaca L.) cultivars native to China were determined by the S-allele PCR approach and the results were confirmed by cross-pollination tests among these cultivars. Primer combination EM-PC2consFD + EM-PC3consR, based on the conserved regions C2 and C3 of Rosaceous S-RNase genes, was the ...
Lijie Zhang +8 more
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A homozygous S genotype of Brassica oleracea expresses two S-like genes
Molecular and General Genetics MGG, 1989The sporophytic self-incompatibility system of Brassica species is controlled by a single locus, S. Recognition of self between pollen and stigma is probably mediated by S locus-specific glycoproteins (SLSGs). We describe the isolation, from an S29 homozygote of Brassica oleracea, of two different cDNA clones for transcripts which are equally abundant ...
M, Trick, R B, Flavell
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Identification of S-genotypes in Chinese cherry cultivars (Prunus pseudocerasus Lindl.)
Tree Genetics & Genomes, 2010Self-incompatibility has been studied extensively at the molecular level in Solanaceae, Rosaceae, and Scrophulariaceae, all of which exhibit gametophytic self-incompatibility. In the present study, we successfully isolated nine S-RNase alleles from cultivars of Chinese cherry by PCR amplification from genomic DNA and stylar cDNA combining with cleaved ...
Chao Gu +6 more
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S-RNase based S-genotyping of Croatian sweet cherry (Prunus avium L.) genotypes
Scientia Horticulturae, 2012Sweet cherries express self-incompatibility, which affects the choice of parents and mating combination in a breeding program. Self-incompatibility is controlled by a multi-allelic single locus (S-locus). Here, the S-alleles were first time studied in 37 local sweet cherry genotypes native to Croatia by using polymerase chain reactions (PCR).
ERCİŞLİ, Sezai +5 more
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PCR-based method for identifying the S-genotypes of Japanese pear cultivars
Theoretical and Applied Genetics, 1999Japanese pear (Pyrus pyrifolia Nakai), a member of the Rosaceae, shows gametophytic self-incompatibility that is controlled by the S-locus. The S-genotype of Japanese pear cultivars is an important factor for crossing and breeding. We report a rapid reliable method to identify these S-genotypes. It consists of PCR amplification of the S-RNase gene from
T. Ishimizu +5 more
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[Identifying the S genotypes of sweet cherry (Prunus avium L.) cultivars].
Yi chuan xue bao = Acta genetica Sinica, 2005This report identified S-RNase genes (S genes) of sweet cherry (Prunus avium L.), presented the sequences of S genes by using a pair of specific primers PruC2 and PruC4R based on the conserved regions C2 and RC4 of Rosaceous S-RNase genes and researched the S gene specific products from the genomic DNAs of different cultivars in which most of the S ...
Xiao-Liu, Chen +2 more
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S-genotyping of sweet cherry varieties from Spain and S-locus diversity in Europe
Euphytica, 2014Sweet cherry is a traditional fruit crop in Spain. The introduction of modern cultivars is replacing the local varieties and genetic diversity is lost. To conserve this plant material, local varieties are being collected and characterized. As part of this objective we investigated the S-genotype of 73 local varieties.
Ariana M. Cachi, Ana Wünsch
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Identification of S-genotypes in almond progenies by NEPHGE and PCR
2012Almond (P. amygdalus Batsch) shows a gametophytic self-incompatibility system controlled by a multiallelic locus, known as the locus S. Self-compatibility has been related to Sf allele presence and this trait has become a priority in the main almond breeding programs and the search for new cultivars is focused on the evaluation of desirable traits in ...
Kamali, K. +4 more
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