Results 91 to 100 of about 432,483 (291)

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source

Calibration of the light-flavour jet mistagging efficiency of the b-tagging algorithms with Z+jets events using 139 $$\textrm{fb}^{-1}$$ of ATLAS proton–proton collision data at $$\sqrt{s} = 13$$ TeV [PDF]

, 2023
G. Aad, B. Abbott, D. C. Abbott, K. Abeling, S. H. Abidi, A. Aboulhorma, H. Abramowicz, H. Abreu, Y. Abulaiti, A. C. Abusleme Hoffman, B. S. Acharya, C. Adam Bourdarios, L. Adamczyk, L. Adámek, S. V. Addepalli, J. Adelman, A. Adıgüzel, S. Adorni, T. Adye, A. A. Affolder, Y. Afik, M. N. Agaras, J. Agarwala, A. Aggarwal, C. Agheorghiesei, J. A. Aguilar–Saavedra, A. Ahmad, F. Ahmadov, W. S. Ahmed, S. Ahuja, X. Ai, G. Aielli, M. Ait Tamlihat, B. Aitbenchikh, I. Aizenberg, M. Akbiyik, T. P. A. Åkesson, A. V. Akimov, K. Al Khoury, G. L. Alberghi, J. Albert, P. Albicocco, S. Alderweireldt, M. Aleksa, I. N. Aleksandrov, C. Alexa, T. Alexopoulos, A. Alfonsi, F. Alfonsi, M. Alhroob, B. Ali, S. Ali, M. Aliev, G. Alimonti, W. Alkakhi, C. Allaire, B. M. M. Allbrooke, C. Flores, P. P. Allport, A. Aloisio, F. Alonso, C. Alpigiani, M. Alvarez Estevez, A. Álvarez Fernández, M. G. Alviggi, M. Aly, Y. Amaral Coutinho, A. Ambler, C. Amelung, M. Amerl, C. G. Ames, D. Amidei, S. P. Amor Dos Santos, K. R. Amos, V. Ananiev, C. Anastopoulos, T. Andeen, J. K. Anders, S. Y. Andrean, A. Andreazza, S. Angelidakis, A. Angerami, A. V. Anisenkov, A. Annovi, C. Antel, M. T. Anthony, E. Antipov, M. Antonelli, D. J. A. Antrim, F. Anulli, M. Aoki, T. Aoki, J. A. Aparisi Pozo, M. A. Aparo, L. Aperio Bella, C. Appelt, N. Aranzabal, V. Araujo Ferraz, C. Arcangeletti, A. T. H. Arce   +99 more
openalex   +1 more source

The anti‐CRISPR protein AcrIE8.1 inhibits the type I‐E CRISPR‐Cas system by directly binding to the Cascade subunit Cas11

FEBS Letters, EarlyView.
In this study, we present the structure of AcrIE8.1, a previously uncharacterized anti‐CRISPR protein that inhibits the type I‐E CRISPR‐Cas system. Through a combination of structural and biochemical analyses, we demonstrate that AcrIE8.1 directly binds to the Cas11 subunit of the Cascade complex to inhibit the CRISPR‐Cas system.
Young Woo Kang, Hyun Ho Park
wiley   +1 more source

An automated method for high-throughput protein purification applied to a comparison of His-tag and GST-tag affinity chromatography

BMC Biotechnology, 2003
Background Functional Genomics, the systematic characterisation of the functions of an organism's genes, includes the study of the gene products, the proteins. Such studies require methods to express and purify these proteins in a parallel, time and cost
Büssow Konrad, Sievert Volker, Scheich Christoph   +2 more
doaj   +1 more source

Transitions between homophilic and heterophilic modes of cooperation

, 2015
Cooperation is ubiquitous in biological and social systems. Previous studies revealed that a preference toward similar appearance promotes cooperation, a phenomenon called tag-mediated cooperation or communitarian cooperation.
Bersini, Hugues, Ichinose, Genki, Saito, Masaya, Sayama, Hiroki   +3 more
core   +1 more source

An intracellular transporter mitigates the CO2‐induced decline in iron content in Arabidopsis shoots

FEBS Letters, EarlyView.
This study identifies a gene encoding a transmembrane protein, MIC, which contributes to the reduction of shoot Fe content observed in plants under elevated CO2. MIC is a putative Fe transporter localized to the Golgi and endosomal compartments. Its post‐translational regulation in roots may represent a potential target for improving plant nutrition ...
Timothy Mozzanino, Meijie Li, Cécile Fizames, Mattia Adamo, Laurence Lejay, Christian Dubos, Matthieu Platre, Antoine Martin   +7 more
wiley   +1 more source

Distributional, differential and integral problems: equivalence and existence results

Electronic Journal of Qualitative Theory of Differential Equations, 2017
We are interested in studying the matter of equivalence of the following problems: \[ \begin{aligned} Dx&=f(t,x)Dg\nonumber \\ x(0)&=x_0 \end{aligned}\tag{1} \label{1a} \] where $Dx$ and $Dg$ stand for the distributional derivatives of $x$ and $g ...
Giselle Monteiro, Bianca Satco
doaj   +1 more source

The Cowl - v.19 - n.19 [2] - Apr 03, 1957 [PDF]

, 1957
The Cowl - student newspaper of Providence College. Volume 19, Number 19 [2] - April 3, 1957. 6 pages. Note:This is the second No.

core   +1 more source

A Cre‐dependent lentiviral vector for neuron subtype‐specific expression of large proteins

FEBS Letters, EarlyView.
We designed a versatile and modular lentivector comprising a Cre‐dependent switch and self‐cleaving 2A peptide and tested it for co‐expression of GFP and a 2.8 kb gene of interest (GOI) in mouse cortical parvalbumin (PV+) interneurons and midbrain dopamine (TH+) neurons.
Weixuan Xue, Sandrine Picaud, Régine Hepp, Stéphanie Pons, Uwe Maskos, Bertrand Lambolez, Ludovic Tricoire   +6 more
wiley   +1 more source

An ER Protein Functionally Couples Neutral Lipid Metabolism on Lipid Droplets to Membrane Lipid Synthesis in the ER

Cell Reports, 2014
Eukaryotic cells store neutral lipids such as triacylglycerol (TAG) in lipid droplets (LDs). Here, we have addressed how LDs are functionally linked to the endoplasmic reticulum (ER). We show that, in S. cerevisiae, LD growth is sustained by LD-localized
Daniel F. Markgraf, Robin W. Klemm, Mirco Junker, Hans K. Hannibal-Bach, Christer S. Ejsing, Tom A. Rapoport   +5 more
doaj   +1 more source