Results 171 to 180 of about 1,785,581 (311)

CryoBN‐PAGE: A Rapid and Cost‐Effective Method for the Analysis of Yeast Respiratory Complexes and Their Dynamic Organization

Yeast, EarlyView.
We present cryoBN‐PAGE, a method that enables blue native polyacrylamide gel electrophoresis analysis of mitochondrial respiratory complexes and supercomplexes directly from whole yeast cells using cryogenic milling. This approach eliminates the need for mitochondrial isolation, reducing time and cost while preserving native protein complexes.
Lizeth J. Camacho Lopez, Mazzen H. Eldeeb, Elliana G. Mohrhardt, Flavia Fontanesi   +3 more
wiley   +1 more source

Genomic signatures of dairy adaptation in <i>Saccharomyces cerevisiae</i> from traditional Yaghnob goat-cheese fermentation. [PDF]

Proc Natl Acad Sci U S A
Bambi M, Goutbi AE, Di Paola M, Bellini M, Cerasuolo B, Diroma MA, Mattarelli P, Nenciarini S, Natali C, Panaino ACD, Ramazzotti M, Stefanini I, Cavalieri D.   +12 more
europepmc   +1 more source

Mapping Allergen B‐ and T‐Cell Epitopes: Technological Advances and Their Role in Precision Allergy Therapy

Allergy, EarlyView.
ABSTRACT Allergic diseases arise from aberrant immune recognition of otherwise harmless environmental proteins and are driven by epitope‐specific interactions between allergens and the adaptive immune system. Although component‐resolved diagnostics have improved molecular characterisation of sensitization, they remain limited to whole‐allergen ...
Mark Møiniche, Josefine Corneliussen, Kristoffer H. Johansen, Alexandre Ruiz‐Carrasco, Caroline Paulsen, Yuchen Li, Carolina Barra, Sandhya Bangaru, Monica L. Fernández‐Quintero, Ewa Bartko, Lars Blom, Esperanza Rivera‐de‐Torre   +11 more
wiley   +1 more source

RcAlb-PepII Perturbs the Proteomic Profile of <i>Cryptococcus neoformans</i>, Shutting Down Proteins Involved in Fungal Survival. [PDF]

Microorganisms
Filho NSDS, Silva L, Cordeiro RA, Lima PG, Neto NADS, Lima PVDR, Gomes FIR, Mororó JLT, Filho JHA, Mesquita FP, Souza PFN.   +10 more
europepmc   +1 more source

Compartmentalisation in cAMP signalling: A phase separation perspective

British Journal of Pharmacology, EarlyView.
Cells rely on precise spatiotemporal control of signalling pathways to ensure functional specificity. The compartmentalisation of cyclic AMP (cAMP) and protein kinase A (PKA) signalling enables distinct cellular responses within a crowded cytoplasmic space.
Milda Folkmanaite, Manuela Zaccolo
wiley   +1 more source

Integrated Physiological and Transcriptomic Analyses of <i>Saccharomyces cerevisiae</i> Under Syringaldehyde Stress. [PDF]

Biology (Basel)
Long X, Li X, Zhao X, Du Y, Niu F, Yi Y.
europepmc   +1 more source

Virtual cell construction for artificial intelligence‐driven drug discovery

British Journal of Pharmacology, EarlyView.
Cells are the fundamental units through which genetic variation and pharmacological perturbations influence disease processes and therapeutic responses. However, cellular responses to intervention are strongly shaped by biological context, creating a central challenge for drug discovery: predicting how specific perturbations reshape cellular systems ...
Yuran Jia, Xiao Xing, Haoyang Han, Yuansong Zhao, Shiyao Zhou, Jingjing Chen   +5 more
wiley   +1 more source

A multi-omics systems toxicology framework reveals amino acid-mediated stress adaptation to deltamethrin exposure in a yeast model. [PDF]

Sci Rep
Yavuz M, Yavuz HG, Oksuzoglu AD, Bereketoglu C, Mardinoglu A, Calimlioglu B.   +5 more
europepmc   +1 more source

Genetic dissection reveals distinct contributions of the eS31 N‐terminal domain to translational accuracy in Saccharomyces cerevisiae

The FEBS Journal, EarlyView.
The eukaryote‐specific N‐terminal domain (NTD) of eS31 uses two distinct strategies to maintain translation fidelity. During elongation, a positively charged “hotspot” fine‐tunes the selection of incoming aa‐tRNA. During termination, the entire NTD acts as a structural scaffold to ensure the correct positioning of the release factor eRF1.
Qingxuan Gao, Hiroshi Otsuka, Kei Endo, Koichi Ito   +3 more
wiley   +1 more source

The G-patch protein Cwf28 interacts with the RNA helicase Cdc28 in catalytically active spliceosomes in Schizosaccharomyces pombe. [PDF]

PLoS One
Karika LO, Cipakova I, Kretova M, Selicky T, Cmikova N, Nemcekova D, Kohutova L, Barath P, Cipak L.   +8 more
europepmc   +1 more source