A general model for analysis of linear and hyperbolic enzyme inhibition mechanisms
FEBS Open Bio, EarlyView.We developed a general enzyme kinetic model that integrates these six basic inhibition mechanism onto a single one. From this model, we deduced a general enzyme kinetic equation that through modulation of simple parameters, γ (the relative inhibitor affinity for two binding sites) and β (the reactivity of the enzyme–substrate–inhibitor complex), is ...
Rafael S. Chagas, Sandro R. Marana
wiley +1 more source
Nanostructures as indicator for deformation dynamics. [PDF]
Nat CommunIncel S +4 more
europepmc +1 more source
HSP70 governs permeability and mechanotransduction in primary human endothelial cells
FEBS Open Bio, EarlyView.HSP70 chemical inhibition reduces endothelial cell proliferation and increases permeability, the latter supported by normal interendothelial junctional protein distribution. HSP70 also plays a role in shear stress response, a hemodynamic force naturally present in blood vessels and correlated with vessel protection.
Andrea Pinto‐Martinez +5 more
wiley +1 more source
Non-invasive estimation of material properties of normal and dissected human ascending aortas <i>in vivo</i>: comparison with the <i>ex vivo</i> tensile experiment. [PDF]
Front Bioeng BiotechnolGuo X, Yang Y, Wang L, Tang D, Sun H.
europepmc +1 more source
Homologous expression and purification of human HAX‐1 for structural studies
FEBS Open Bio, EarlyView.This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley +1 more source
Effects of Post-Heat Treatment on Mechanical and Tribological Properties of 3D-Printed PLA and PEEK Structures. [PDF]
Polymers (Basel)Deng Y, Chang L.
europepmc +1 more source
Purification of genetic material concentration from dental samples using EBiS assisted with MNPS
Mara Yatzín Alcántar-Jacobo +6 more
openalex +1 more source
Thrombolytic proteins profiling: High‐throughput activity, selectivity, and resistance assays
FEBS Open Bio, EarlyView.We present optimized biochemical protocols for evaluating thrombolytic proteins, enabling rapid and robust screening of enzymatic activity, inhibition resistance, and fibrin affinity, stimulation, and selectivity. The outcome translates to key clinical indicators such as biological half‐life and bleeding risk. These assays streamline the development of
Martin Toul +3 more
wiley +1 more source
Effect of Bead Geometry and Layer Time on Microstructure and Thermomechanical Properties of Large-Format Polymer Composites. [PDF]
Polymers (Basel)Corum TM +5 more
europepmc +1 more source