Results 161 to 170 of about 2,242,200 (261)

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Erratum for CySP3-96 Enables Scalable, Streamlined, and Low-Cost Sample Preparation for Cysteine Chemoproteomic Applications. [PDF]

Mol Cell Proteomics
europepmc   +1 more source

Micron-resolution fiber mapping in histology independent of sample preparation. [PDF]

Nat Commun
Georgiadis M, Auf der Heiden F, Abbasi H, Ettema L, Nirschl J, Taghavi HM, Wakatsuki M, Liu A, Ho WHD, Carlson M, Doukas M, Koppes SA, Keereweer S, Sobel RA, Setsompop K, Liao C, Amunts K, Axer M, Zeineh M, Menzel M.   +19 more
europepmc   +1 more source

Nuclear pore links Fob1‐dependent rDNA damage relocation to lifespan control

FEBS Open Bio, EarlyView.
Damaged rDNA accumulates at a specific perinuclear interface that couples nucleolar escape with nuclear envelope association. Nuclear pores at this site help inhibit Fob1‐induced rDNA instability. This spatial organization of damage handling supports a functional link between nuclear architecture, rDNA stability, and replicative lifespan in yeast.
Yamato Okada, Mina Iwaki, Kyosuke Hagiri, Rei Izumi, Masahiko Harata, Chihiro Horigome   +5 more
wiley   +1 more source

High-Throughput Proteomics Sample Preparation Using a 96-Channel Pipettor and Magnetic Pin Device. [PDF]

J Proteome Res
Roumelioti G, Montoya A, Fisher GLM, Navarro EP, Woods A, Bennett J, Navaratnam N, Gonzalez-Carvajal O, Birch J, Pyman E, Yu S, Gruevska A, Vuillemenot LA, Lushchak O, Hall Z, Barr AR, Speck C, Vernia S, Scott WR, Gil J, Aragon L, Fets L, Carling D, Shliaha PV.   +23 more
europepmc   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Shake and bake: a robust and cost-effective proteomic sample preparation workflow for plasma and cerebrospinal fluid. [PDF]

Clin Proteomics
Rönnholm J, Weiner S, Fuchs J, Thorsell A, Sihlbom C, Dugom L, Easton A, Zetterberg H, Gobom J.   +8 more
europepmc   +1 more source

Promiscuous stimulation of HSP70 ATPase activity by parasite‐derived J‐domains

FEBS Open Bio, EarlyView.
The malaria parasite Plasmodium falciparum exports three highly homologous yet functionally divergent J‐domain proteins into human erythrocytes. Here, we show that J‐domains isolated from all three proteins effectively stimulate the ATPase activity of both endogenous host and exported parasite HSP70 chaperones.
Julian Barth, Moritz Koch, Le‐Han Rössner, Johanna Eichhorn, Denys Pogoryelov, Matthias P. Mayer, Jude M. Przyborski   +6 more
wiley   +1 more source

Erythropoietin modulates hepatic inflammation, glucose homeostasis, and soluble epoxide hydrolase and epoxides in high‐fat diet‐induced obese mice

FEBS Open Bio, EarlyView.
Erythropoietin administration suppresses hepatic soluble epoxide hydrolase (sEH) expression, leading to increased CYP‐derived epoxides. This is associated with a shift in hepatic macrophage polarization characterized by reduced M1 markers and increased M2 markers, along with reduced hepatic inflammation, suppressed hepatic lipogenesis, and attenuated ...
Takeshi Goda, Satoru Sugimoto, Chiharu Cho, Madoka Konishi, Nozomi Inoue, Satoshi Miyagaki, Yasuhiro Kawabe, Takuro Okamura, Masahide Hamaguchi, Hisakazu Nakajima, Michiaki Fukui, Masakazu Shinohara, Tomoko Iehara   +12 more
wiley   +1 more source

Impact of Preanalytical Sample Preparation on the Recovery Rate of Cryopreserved Allogeneic Hematopoietic Stem Cells. [PDF]

Eur J Haematol
Blüm P, Glas C, Bieback K, Kayser S, Klüter H, Wuchter P.   +5 more
europepmc   +1 more source