Results 201 to 210 of about 2,377,512 (309)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Investigation of Sample Preparation Methods of Dietary Supplements for Total Selenium Determination. [PDF]

ACS Omega
Oliveira LMA, Moraes DP, Pereira JSF.
europepmc   +1 more source

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

Spatial Imaging and Quantification of Hydrogen Peroxide in <i>Arabidopsis</i> Roots: From Sample Preparation to Image Analysis. [PDF]

Bio Protoc
Fenech M, Amorim-Silva V.
europepmc   +1 more source

Sample Preparation and Exotic Magnetic Properties of High Tc N-type Superconductor Nd1.85 CuO4-σ [PDF]

, 2014
G. H. Hwang, H. C. Ku., C. Y. Lin, T. H. Her   +3 more
core  

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Toward Sustainable Clinical Analysis: Benchmarking Plastic Use in LC-MS Sample Preparation - Exemplified by Ketamine Analogues in Whole Blood. [PDF]

Anal Chem
Noreng L, Øiestad ÅML, Hansen FA, Røberg-Larsen H, Wilson SR, Øiestad EL.   +5 more
europepmc   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Micron-resolution fiber mapping in histology independent of sample preparation. [PDF]

Nat Commun
Georgiadis M, Auf der Heiden F, Abbasi H, Ettema L, Nirschl J, Taghavi HM, Wakatsuki M, Liu A, Ho WHD, Carlson M, Doukas M, Koppes SA, Keereweer S, Sobel RA, Setsompop K, Liao C, Amunts K, Axer M, Zeineh M, Menzel M.   +19 more
europepmc   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source