Results 81 to 90 of about 191 (111)
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Plant Physiology and Biochemistry, 2006
Chalcone isomerase (CHI; EC 5.5.1.6) is a key enzyme in the flavonoid biosynthesis pathway. We isolated a CHI gene (SmCHI) from a cDNA library derived from Saussurea medusa (Asteraceae) cell cultures. The cDNA and genomic sequences of SmCHI are the same; in other words, this gene is intronless.
F, Li, Z, Jin, W, Qu, D, Zhao, F, Ma
exaly +3 more sources
Chalcone isomerase (CHI; EC 5.5.1.6) is a key enzyme in the flavonoid biosynthesis pathway. We isolated a CHI gene (SmCHI) from a cDNA library derived from Saussurea medusa (Asteraceae) cell cultures. The cDNA and genomic sequences of SmCHI are the same; in other words, this gene is intronless.
F, Li, Z, Jin, W, Qu, D, Zhao, F, Ma
exaly +3 more sources
Plant Cell Reports, 2003
Cell suspension cultures of Saussurea medusa were grown in shake flasks and a 5-l stirred tank bioreactor. Biomass and jaceosidin distribution in cell aggregates of different sizes were investigated during the cultivation period. The results showed that on day 10, jaceosidin accumulation showed an increase with increasing size of the cell aggregate to ...
D, Zhao, Y, Huang, Z, Jin, W, Qu, D, Lu
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Cell suspension cultures of Saussurea medusa were grown in shake flasks and a 5-l stirred tank bioreactor. Biomass and jaceosidin distribution in cell aggregates of different sizes were investigated during the cultivation period. The results showed that on day 10, jaceosidin accumulation showed an increase with increasing size of the cell aggregate to ...
D, Zhao, Y, Huang, Z, Jin, W, Qu, D, Lu
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[Cloning, expression and charaterization of chalcone synthase from Saussurea medusa].
Sheng wu gong cheng xue bao = Chinese journal of biotechnology, 2013A fragment of chalcone synthase gene (SmCHS) was cloned from the cDNA library constructed in Saussurea medusa. The full-length cDNA sequence of SmCHS was obtained by RT-PCR. Sequence analysis showed that the full length of SmCHS was 1313 bp, containing an open reading frame (1170 bp) encoding 389 amino acids.
Fang, Xia +5 more
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Biotechnology Letters, 2005
Three previously established cell lines (yellow, red and white) of Saussurea medusa were investigated for jaceosidin and hispidulin production. Maximum yields of the jaceosidin and hispidulin were obtained in the red cell line at 75+/-0.41 and 6.4+/-0.31 mg l-1.
Chun-xiang, Fu +3 more
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Three previously established cell lines (yellow, red and white) of Saussurea medusa were investigated for jaceosidin and hispidulin production. Maximum yields of the jaceosidin and hispidulin were obtained in the red cell line at 75+/-0.41 and 6.4+/-0.31 mg l-1.
Chun-xiang, Fu +3 more
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Plant Cell, Tissue and Organ Culture, 2001
Calli cultures derived from the leaves of Saussurea medusa were selected on the basis of colour into three callus, A, B and C, which suggested different levels of metabolite accumulation. An improved reversed phase high performance liquid chromatographic method provided selective determination of the jaceosidin content of these samples.
Dexiu Zhao +4 more
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Calli cultures derived from the leaves of Saussurea medusa were selected on the basis of colour into three callus, A, B and C, which suggested different levels of metabolite accumulation. An improved reversed phase high performance liquid chromatographic method provided selective determination of the jaceosidin content of these samples.
Dexiu Zhao +4 more
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Process Biochemistry, 2005
Abstract Suspension cultures of Sussurea medusa produce jaceosidin and hispidulin. In an attempt to increase productivity, silver nitrate and glutathione (GSH) elicitors were tested. Both dosage and addition time as well as time course studies were carried out on the effects of the elicitors on the formation of the metabolites.
De-Xiu Zhao +3 more
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Abstract Suspension cultures of Sussurea medusa produce jaceosidin and hispidulin. In an attempt to increase productivity, silver nitrate and glutathione (GSH) elicitors were tested. Both dosage and addition time as well as time course studies were carried out on the effects of the elicitors on the formation of the metabolites.
De-Xiu Zhao +3 more
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Cloning and expression analysis of a hsp70 gene from Saussurea medusa.
DNA sequence : the journal of DNA sequencing and mapping, 2006Members of the 70 kD heat-shock gene family are highly conserved across a wide range of organisms. In an effort to learn more about the evolution and possible functions of extreme environment plant Saussurea medusa Maxim hsp70, we isolated a cDNA clone encoding a putative cytosolic member (Smhsp70) of this family of proteins from a cDNA library of S ...
Liqin, Cheng +3 more
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[Cloning of flavone synthase (FNSII) gene and expression in three cell lines of Saussurea medusa].
Sheng wu gong cheng xue bao = Chinese journal of biotechnology, 2016Saussurea medusa is a rare traditional Chinese medicinal herb, of which luteolin is the niain active medicinal compound for cancer prevention and treatment. A full-length FNSII gene, namely SmFNSII (GenBank Accession No. KF170286), was obtained from green cell line of Saussurea medusa by RT-PCR and RACE-PCR.
Bingjie, Wang +5 more
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Chemical Constituents of Saussurea medusa
Chemistry of Natural Compounds, 2023Nan Wu, Ruitao Yu
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Heart, 2013
Objectives To investigate the effects of the cell lines of saussurea medusa (CLSM) on regulating blood lipid in rats with hyperlipidemia. Methods A randomised controlled experimental Study.
Yang Bo, Chen Yundai, Li Min
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Objectives To investigate the effects of the cell lines of saussurea medusa (CLSM) on regulating blood lipid in rats with hyperlipidemia. Methods A randomised controlled experimental Study.
Yang Bo, Chen Yundai, Li Min
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