Results 141 to 150 of about 2,619,148 (295)

Evolution‐guided yeast complementation reveals functional differences in human PSPH variants

FEBS Open Bio, EarlyView.
Ancient genomes can help guide which human genetic variants are tested experimentally. This study applies that idea to PSPH, a gene involved in serine biosynthesis, and uses high‐throughput yeast complementation to compare variant function. The findings reveal measurable differences among selected alleles and illustrate the value of evolution‐guided ...
Mauricio Campa‐Álvarez, Diana Ascencio, Miguel Vallebueno‐Estrada, Eduardo González‐Orozco, Christian Eduardo Martínez‐Guerrero, Rafael Montiel, Alexander DeLuna   +6 more
wiley   +1 more source

Pattern-driven reuse in architecture-centric evolution for service software [PDF]

, 2012
Service-based architectures implement business processes as technical software services to develop enterprise software. As a consequence of frequent business and technical change cycles, the architect requires a reusecentered approach to systematically ...
Pooyan Jamshidi (5276344), Ahmad, Aakash, Claus Pahl (12588787), Pahl, Claus, Jamshidi, Pooyan, Aakash Ahmad (5022314)   +5 more
core   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

A Low Power Test Pattern Generation Methodology for Scan Testing

, 2007
平均與最高功率管理在現行掃描鏈測試中成為一個嚴重的議題，測試IC時會產生比一般工作情況下更大的功率消耗，本篇論文提出一個新的自動測試圖樣產生技術，目標在降低測試功率消耗，此技術同時考慮移入(shift)與捕捉(capture)時之平均與最高功率消耗。 此技術整合一功率限制之自動測試圖樣產生引擎，一功率限制之動態測試向量壓縮機制，一未指定值位元填充(X-filling)方法，與一重新安排測試向量順序方法，以達到降低功率之目的。此外，本論文提出一機制，將此降低測試功率技術與現行商業自動測試圖樣產生器連接 ...
胡凱舜, Hu, Kai-Shun
core  

Avidin is evolutionarily conserved in fish but dispensable for development and resistance against Streptococcus agalactiae in zebrafish

FEBS Open Bio, EarlyView.
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti, Markus J. T. Ojanen, Mataleena Parikka, Otto Kauko, Mika Rämet, Vesa P. Hytönen   +5 more
wiley   +1 more source

GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma

FEBS Open Bio, EarlyView.
GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain, Elise Buret, Le Hoang Thanh Nguyen, Marion Cocheteux, Nicolas Touya, Mélanie Dhayer, Romain Dubois, Frank Lafont, Sebastien Janel, Patrice Maboudou, Steve Lancel, Philippe Marchetti   +11 more
wiley   +1 more source

Very Easy Very Vogue

, 1988
Very Easy Vogue Career Pattern #7382 to make a straight dress with shoulder pads, front overlay, back zipper, and long set-in sleeves, including illustrations of the finished pieces and information about sizing and construction on the ...
Vogue Pattern
core  

Structural and biochemical insights into the thermostable esterase Ta0887 from Thermoplasma acidophilum

FEBS Open Bio, EarlyView.
In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey, Miriam Livier Llamas‐García, Gabriela M. Montero‐Morán, Leticia Santos, Samuel Lara‐González   +4 more
wiley   +1 more source

Impact of Optical Coherence Tomography Scan Pattern and Chorio-Scleral Interface Delineation on Choroidal Vascularity Index Measurements. [PDF]

Ophthalmic Physiol Opt
Wong KYQ, Chun RKM, Alonso-Caneiro D, Chan YP, Lam AKC.   +4 more
europepmc   +1 more source