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High levels of Cdc42 GTPase underlie an all-or-none decision to fuse
Saha S +4 more
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The oncogene SLC35F2 is a high-specificity transporter for the micronutrients queuine and queuosine
Burtnyak L +15 more
europepmc +1 more source
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Vectors for the expression of tagged proteins in Schizosaccharomyces pombe
Gene, 1998A series of vectors is described which enables the episomal expression of proteins fused to different tag sequences in Schizosaccharomyces pombe. Proteins can be expressed with their amino termini fused to GFP/EGFP, three copies of the HA or Pk epitopes or a combined tag which contains two copies of the myc epitope and six histidine residues (MH ...
Craven, R A +5 more
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The ypt proteins of Schizosaccharomyces pombe
Biochemical Society Transactions, 199465 66 Mayer, B. J., Ren, K., Clark, K. I,. and Baltimore, D. (1993) Cell 73,629-630 Haslarn, K. J., Koide, H. B. and Hemmings, B. A. (1993) Nature (London) 363,309-310 Musacchio, A., Gibson, T., Price, P., Thompson, J. and Saraste, M. (1993) Trends Biochem. Sci. 18, 343-348 Lefkowitz, R. J. (1993) Cell 74,409-412 Bourne, H. K., Sanders, D. A.
Everton Robinson +5 more
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Characterization of Schizosaccharomyces pombe secreted proteins
HEREDITAS, 2007Secreted proteins play a vital physiological role. Schizosaccharomyces pombe is an important model organism for cell cycle study and a potential useful drug screen model. Secreted proteins also initiate the mating. However, few global studies concerning the secreted proteins of S. pombe was reported.
Yun-Fan Liu, Yu-Ling Liu, Jian-Ping Xie
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Protein transport in the permeabilized cell of Schizosaccharomyces pombe
Biochemical and Biophysical Research Communications, 1992We reconstituted a protein translocation-transport system composed of permeabilized spheroplasts (P-cells) of the fission yeast Schizosaccharomyces pombe and the precursor of alpha sex pheromone, prepro-alpha-factor of the budding yeast Saccharomyces cerevisiae.
Koji Yoda +2 more
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Recombinational repair in Schizosaccharomyces pombe: The role of mediator proteins
Molecular Biology, 2012Repair of double-stranded DNA breaks that occur spontaneously or under the influence of external factors, are critical for cell survival. Evolutionarily conserved mechanism for error-free recombinational repair plays a major role in maintaining genome integrity.
D. A. Vagin +2 more
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Identification of two forms of the Eso1 protein in Schizosaccharomyces pombe
Cell Biology International, 2014AbstractIn Schizosaccharomyces pombe, Eso1p is a protein fusion. Two‐thirds of its N‐terminus is conserved to budding yeast Rad30, which functions in error‐free replication of UV‐damaged DNA. A third of the C‐terminus is highly conserved to budding yeast Eco1, a lysine acetyltransferase, which is essential for the establishment of cohesion. Both Rad30p
Yingqiang Lu +3 more
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Using Schizosaccharomyces pombe as a host for expression and purification of eukaryotic proteins
Gene, 1997We have established a eukaryotic protein expression and purification system by using the yeast Schizosaccharomyces pombe as the host and the glutathione S-transferase (GST) as a protein purification tag. This system provides opportunities for rapid, inexpensive, and high yield production of proteins in a eukaryotic organism. Unlike E. coli, S.
Quinn Lu +2 more
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