Results 271 to 280 of about 56,565 (308)
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Schizosaccharomyces pombe fragile mutants as a host for heterologous protein production
Journal of Biotechnology, 1997In order to gain information about the potential interest of the Schizosaccharomyces pombe srb 1 fragile mutants as a host for heterologous protein production, the extracellular secretion of homologous and heterologous invertases was investigated. Under catabolic derepression the fragile srb 1 mutants released into the extracellular medium 5-6-fold ...
Victoria Zárate, Fernando Belda
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CSL proteins of Schizosaccharomyces pombe
2009The CSL family of transcription factors is essential for metazoan development,mostly due to their involvement in the Notch signaling pathway. We identified two novel classes of CSL genes in several fungal species, organisms lacking the Notch pathway. We characterized experimentally cbfl I- and cbfl2*, the two CSL genesof Schizosaccharomyces pombe, in ...
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A cDNA of Schizosaccharomyces pombe encoding a homologue of DnaJ-like protein
Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1995A Schizosaccharomyces pombe homologue, Psi, was cloned from Schizosaccharomyces pombe cDNA library. Deduced amino acid sequence of the cDNA has 55% sequence homology with the Saccharomyces cerevisiae Sis1 protein and contains the structural features of a family of DnaJ proteins.
Seung Kiel Park +2 more
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Biochemical and Biophysical Research Communications, 2003
Intracellular vesicle trafficking is mediated by a set of SNARE proteins in eukaryotic cells. Several SNARE proteins are required for vacuolar protein transport and vacuolar biogenesis in Saccharomyces cerevisiae. A search of the Schizosaccharomyces pombe genome database revealed a total of 17 SNARE-related genes.
Naotaka Tanaka +5 more
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Intracellular vesicle trafficking is mediated by a set of SNARE proteins in eukaryotic cells. Several SNARE proteins are required for vacuolar protein transport and vacuolar biogenesis in Saccharomyces cerevisiae. A search of the Schizosaccharomyces pombe genome database revealed a total of 17 SNARE-related genes.
Naotaka Tanaka +5 more
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Expression and Secretion of Mammalian Proteins in Schizosaccharomyces pombe
1997Saccharomyces cerevisiae is a widely used host for the expression of eukaryotic proteins. However, due to problems such as poor recognition of foreign signal sequences, improper processing and poor secretion, alternative eukaryotic expression systems such as Pichia pastoris have been recently developed.1 The fission yeast Schizosaccharomyces pombe ...
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Yeast, 1992
AbstractA technique is described for the isolation and purification of intact, respiratory‐competent mitochondria from Sachizo‐saccharomyces pombe. The purified mitochondria are capabel of oxidizing NADH and succinate as resporatory substrates. indicating the presence of succinate dehydorgenase and an NADH dehydrogenase located on the outer suface of ...
Anthony L. Moore +4 more
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AbstractA technique is described for the isolation and purification of intact, respiratory‐competent mitochondria from Sachizo‐saccharomyces pombe. The purified mitochondria are capabel of oxidizing NADH and succinate as resporatory substrates. indicating the presence of succinate dehydorgenase and an NADH dehydrogenase located on the outer suface of ...
Anthony L. Moore +4 more
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Characterization of the Prk1 protein kinase fromSchizosaccharomyces pombe
Yeast, 1998We report the isolation and characterization of a protein kinase from the fission yeast Schizosaccharomyces pombe. The proposed Prk1 protein contains 352 amino acids and has significant homology to the Ume5p kinase (also known as Srb10p, Ssn3p and Are1p) of the budding yeast Saccharomyces cerevisiae, a cyclin-dependent kinase involved in regulating the
Peter Duncan Watson, John Davey
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Characterization of Nrl1 protein in Schizosaccharomyces pombe
2016The fission yeast Schizosaccharomyces pombe, is an ideal model organism to study eukaryotic cell biology as it is easy to manipulate while sharing many features in common with higher organisms like centromeres with repetitive sequences, large replication origins, conserved telomere proteins, conserved heterochromatin proteins and epigenetic silencing ...
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The first two‐dimensional reference map of the fission yeast, Schizosaccharomyces pombe proteins
Proteomics, 2005N. Sun +10 more
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