Results 61 to 70 of about 2,256,721 (219)

Wasting disease and environmental variables drive sea star assemblages in the northern Gulf of Alaska [PDF]

open access: yes, 2019
Thesis (M.S.) University of Alaska Fairbanks, 2019Sea stars are ecologically important in rocky intertidal habitats. The recent (starting 2013) sea star die-off attributed to sea star wasting disease throughout the eastern Pacific, presumably triggered ...
Mitchell, Timothy James
core  

Directed evolution of enzymes at the crossroads of tradition and innovation

open access: yesFEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova   +2 more
wiley   +1 more source

Ecological causes and consequences of Sea Star Wasting Syndrome on the Pacific coast [PDF]

open access: yes, 2018
Disease outbreaks are becoming more frequent as anthropogenic changes to ecosystem function stress species and create conditions favorable for pathogen infection.
Moritsch, Monica
core   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

open access: yesFEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen   +5 more
wiley   +1 more source

Identification and characterisation of calcitonin receptor isoforms expressed in glioblastoma derived glioma stem and U‐87 MG cells

open access: yesFEBS Open Bio, EarlyView.
Glioblastoma cells express calcitonin receptor variants (CT receptor isoforms) that may help them survive stress. Using qPCR, transcript‐specific long‐read nanopore sequencing, immunofluorescence co‐localisation and comparative sequence analysis, this study identifies a novel alternatively spliced CALCR transcript that encodes the CTb receptor isoform ...
Pragya Gupta   +7 more
wiley   +1 more source

Recent insights into the molecular mechanism of ubiquinol oxidation by cytochrome bc1

open access: yesFEBS Open Bio, EarlyView.
The review reflects on the mechanism of the catalytic reaction in cytochrome bc1: the electron bifurcation that involves the separation of two electrons derived from the quinol oxidation to opposite sides of the enzyme across the membrane. This review summarizes the long‐standing effort to understand the mechanism of quinol oxidation catalyzed by ...
Anna Wójcik‐Augustyn   +2 more
wiley   +1 more source

Identification and characterization of a gene conferring stress resistance in Escherichia coli and cyanobacteria

open access: yesFEBS Open Bio, EarlyView.
Functional screening identified PcSyn14890, a cyanobacteria‐specific protein that enhances growth and stress resistance in E. coli and Synechocystis PCC6803. Although we expected it to function as a molecular chaperone, it was unable to protect against thermal aggregation of GAPDH.
Akiyo Yamada   +7 more
wiley   +1 more source

Determinants of undergraduate bioscience engagement: Validating attendance barriers and classroom spatial behaviour across institutions

open access: yesFEBS Open Bio, EarlyView.
Student engagement extends beyond simple attendance. Across 891 bioscience students from three universities, attendance behaviour was associated mainly with structural influences, whereas classroom spatial behaviour reflected affective and sensory factors.
Nigel Page   +4 more
wiley   +1 more source

Leveraging diverse datasets to assess imperilled species declines: the Sunflower Sea Star in Canada as a case study

open access: yesFACETS
Evaluating the extinction risk of species is critical for establishing conservation priorities, yet efforts to do so are often hindered by a lack of targeted scientific monitoring data. Since myriad new forms of data are available from community-sourced (
Hannah V. Watkins   +9 more
doaj   +1 more source

Sea Star Wasting Syndrome Modeling: Cox Proportional Hazards Data and Code

open access: yes, 2019
Sea Star Wasting Syndrome (SSWS) has been ongoing in echinoderm species of the North American Pacific Coast. Previous outbreaks, as well as the most recent one, have reduced sea 21 star populations to near-absence in many locations. To identify potential
M Moritsch (6437141)
core   +1 more source

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