Results 51 to 60 of about 2,874 (281)

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

An Improved Dual Second-Order Generalized Integrator Phased-Locked Loop Strategy for an Inverter of Flexible High-Voltage Direct Current Transmission Systems under Nonideal Grid Conditions

, 2023
High-voltage flexible power systems, with their intrinsic characteristics, play an increasingly important role in electronic power systems. Synchronization between the inverter and the grid needs to be achieved by a phase-locked loop (PLL), the ...
Lai Peng, Yang Qian, Zhichao Fu, Wei Zhao, Cheng Zhang, Tao Xiao   +5 more
core   +1 more source

3-Point Block Methods for Direct Integration of General Second-Order Ordinary Differential Equations [PDF]

Advances in Numerical Analysis, 2011
A Multistep collocation techniques is used in this paper to develop a 3-point explicit and implicit block methods, which are suitable for generating solutions of the general second-order ordinary differential equations of the form . The derivation of both explicit and implicit block schemes is given for the purpose of comparison of results.
Julius Osato Ehigie, Solomon A. Okunuga, Adetokunbo B. Sofoluwe   +2 more
openaire   +2 more sources

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

PI/PID Controller Relay Experiment Auto-Tuning with Extended Kalman Filter and Second-Order Generalized Integrator as Parameter Estimators

, 2023
This paper presents a method for the estimation of key parameters of limit cycle oscillations (amplitude and frequency) during a relay experiment used for automatic tuning of proportional-integral (PI) and proportional-integral-derivative (PID) feedback ...
Kolar, Davor, Cipek, Mihael, Pavković, Danijel, Lisjak, Dragutin   +3 more
core   +2 more sources

Clustering Algorithm Reveals Dopamine‐Motor Mismatch in Cognitively Preserved Parkinson's Disease

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective To explore the relationship between dopaminergic denervation and motor impairment in two de novo Parkinson's disease (PD) cohorts. Methods n = 249 PD patients from Parkinson's Progression Markers Initiative (PPMI) and n = 84 from an external clinical cohort.
Rachele Malito, Chiara Meneghini, Alice Galli, Luca Gallo, Pierfrancesco Mitrotti, Paola Dimartino, Marianna Inglese, Cinzia Zatti, Alessandro Lupini, Andrea Pilotto, Alessandro Padovani, Micol Avenali, Enza Maria Valente, Cristina Tassorelli, Silvia P. Caminiti   +14 more
wiley   +1 more source

Instantaneous Symmetrical Component Estimator Using Second Order Generalized Integrator under Distorted Voltage Conditions

, 2017
Frequency, amplitude and phase information of the grid voltage are of great importance in constructing a robust controller structure for grid connected inverter systems. This paper presents a simple and robust approach for the instantaneous estimation of
Pierluigi Siano, Rasool Akhtar, Asif Sabanovic, Vincenzo Loia, Sanjeevikumar Padmanaban, Fiaz Ahmad, Emre Ozsoy   +6 more
core   +1 more source

Improved Nonlinear Flux Observer-Based Second-Order SOIFO for PMSM Sensorless Control [PDF]

, 2019
The conventional rotor flux estimation method has issues of dc offset and harmonics, which are caused by initial rotor flux, detection errors, etc. To eliminate these defects, one improved nonlinear flux observer is proposed for sensorless control of ...
Yajie Jiang, Chaoxu Mu, Jiang, Yajie, Wei Xu, Mu, Chaoxu, Blaabjerg, Frede; id_orcid, Frede Blaabjerg, Xu, Wei   +7 more
core   +1 more source

Fluid Biomarkers of Disease Burden and Cognitive Dysfunction in Progressive Supranuclear Palsy

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Identifying objective biomarkers for progressive supranuclear palsy (PSP) is crucial to improving diagnosis and establishing clinical trial and treatment endpoints. This study evaluated fluid biomarkers in PSP versus controls and their associations with regional 18F‐PI‐2620 tau‐PET, clinical, and cognitive outcomes.
Roxane Dilcher, Charles B. Malpas, Stuart J. McDonald, William T. O’Brien, Craig Despott, Kelly L. Bertram, Matthew P. Pase, Lukas Frontzkowski, Meng Law, Terence J. O’Brien, Lucy Vivash   +10 more
wiley   +1 more source

Enhanced Voltage Sensorless Control for a PWM Converter with DSOGI-FLL Under Grid Disturbances

Energies
This paper presents Enhanced Voltage Sensorless Control for PWM converter with DSOGI-FLL under grid disturbances. Even without grid voltage sensors, the proposed method accurately estimates the grid angle and voltage, which are necessary for power ...
Seung-Pyo Kang, Dong-Youn Kim, Jang-Mok Kim   +2 more
doaj   +1 more source