Results 191 to 200 of about 2,941,160 (266)

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

FEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty, Kelsi R. Hall, Zachary D. Tillett, Liam S. Turk, James S. Davies, Michael J. Currie, Hamish G. Brown, Sepideh Valimehr, Peter D. Mace, Andrew E. Whitten, Santosh Panjikar, Eric Hanssen, Rachel A. North, Renwick C.J. Dobson   +13 more
wiley   +1 more source

Preoperative oral carbohydrate administration in adult elective surgery: protocol heterogeneity, regional practice patterns and evidence gaps-a scoping review. [PDF]

Front Nutr
Xiao B, Liu Y, Lu R, Tan R, Zeng X, Lu X, Yang C.   +6 more
europepmc   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Retrospective Evaluation of Surgical Outcomes in Cats With Glaucoma Following Diode Endoscopic Cyclophotocoagulation. [PDF]

Vet Ophthalmol
Perry EN, Galetti M, Hall E, Irving WM, Whittaker C, Caruso K, McCarthy PMG, Bliss C.   +7 more
europepmc   +1 more source

Evaluation of a Mass Media Educational Campaign to Increase Secondary School Students' Awareness of Health Risks of Khat Chewing: A Quasi-Experimental Pre-Post Study in Aden, Yemen. [PDF]

Med Sci Monit
Alkaf MS, Omar M, Basaleem H, Alsabhan JF, Alsharif M, Shubayr MA, Mahfouz MS, Abdalbari MOA, Fadel HT, Alsharif AT, Al Moaleem MM, Kassim S.   +11 more
europepmc   +1 more source

Association between age and outcomes of evidence-based feeding protocol versus usual care in critically ill patients: secondary analysis of a multicenter cluster-randomised controlled trial. [PDF]

J Intensive Care
Wang Q, Ke J, Chen Y, Xu H, Wu D, Ke L, Liang F, Chen Y, Chinese Critical Care Nutrition Trials Group (CCCNTG).   +8 more
europepmc   +1 more source

Impact on Health Disparities: Secondary Analysis of an Unplanned Extubation Quality Improvement Study. [PDF]

Pediatr Qual Saf
Sreenivasan G, Krishnan P, Parton LA, Singh M.   +3 more
europepmc   +1 more source