Results 191 to 200 of about 2,857,626 (267)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Gut microbiome drives glycodeoxycholic acid-mediated attenuation of hypertension. [PDF]

Gut Microbes
Aryal S, Mell B, Tummala R, Manandhar I, Kumariya S, Kondapalli N, Yeoh BS, Ahlidja W, Mautin Akinola O, Pachhain S, Bardhan P, Saha P, Zeydabadinejad S, Osman I, Thodeti C, Yang T, Vijay-Kumar M, Reddivari L, Joe B.   +18 more
europepmc   +1 more source

Computer Control of Secondary Beams [PDF]

, 1974
openaire   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Effects, Acceptability, and Use of a Dynamically Tailored Mobile What Do You Drink Intervention to Reduce Excessive Drinking Among Adolescents and Young Adults in the Netherlands: Randomized Controlled Trial. [PDF]

JMIR Mhealth Uhealth
van Keulen H, Voogt C, Kleinjan M, Huizing A, Andree R, van Empelen P.   +5 more
europepmc   +1 more source

Dual native G‐quadruplex folding is associated with chromatin looping at the MYC locus

FEBS Open Bio, EarlyView.
BG4‐detectable G‐quadruplex (G4) in HaCaT and NHEK keratinocytes identified folded and unfolded G4s enriched at promoters/TSSs and active enhancers, whereas unfolded G4s also overlapped weak/poised enhancers. At MYC–PVT1, 3C‐qPCR detected enhancer–promoter looping only when G4s were simultaneously folded at both regulatory elements under native ...
Dieila Giomo de Lima, Gustavo Narvaes Guimarães, Emilyane de Oliveira Santana Amaral, Bianca Caroline Figueiredo Bianco, Manuel Jara‐Espejo, Sérgio Roberto Peres Line, Ana Paula de Souza, Aline Cristiane Planello   +7 more
wiley   +1 more source

Profiles of career adaptability and their associations with sociodemographic characteristics, big five traits, and mental health among Chinese primary and secondary school teachers. [PDF]

Front Psychol
She C, Zou L, Zhou J.
europepmc   +1 more source

Entomopathogenic Nematode Steinernema rarum and its Symbiotic Bacterium against Fire Ants (Solenopsis sp.) under Laboratory Conditions. [PDF]

Neotrop Entomol
Babesco CE, Chacón-Orozco JG, Leite LG, Harakava R, de Carvalho Campos AE.   +4 more
europepmc   +1 more source