Results 41 to 50 of about 21,809 (264)

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

Additive manufacturing of Ti6Al4V alloy: A review

Materials & Design, 2019
In this paper, the recent progress on Ti6Al4V fabricated by three mostly developed additive manufacturing (AM) techniques-directed energy deposition (DED), selective laser melting (SLM) and electron beam melting (EBM)-is thoroughly investigated and ...
Shunyu Liu, Yung C. Shin
doaj   +1 more source

Laser Polishing of Ti6Al4V Fabricated by Selective Laser Melting

Metals, 2020
Selective laser melting (SLM) is emerging as a promising 3D printing method for orthopedic and dental applications. However, SLM-based Ti6Al4V components frequently exhibit high roughness values and partial surface defects.
Chunyong Liang, Yazhou Hu, Ning Liu, Xianrui Zou, Hongshui Wang, Xinping Zhang, Yulan Fu, Jingyun Hu   +7 more
doaj   +1 more source

A new flow chip in combination with multiphoton microscopy as a protocol for longitudinal 3D imaging of tissue calcification under shear stress

FEBS Open Bio, EarlyView.
Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas, Aaron D. Morgan, Ignas Čiurlionis, Christian Böhm, Stefan Jockenhövel, Willi Jahnen‐Dechent, Marc A. M. J. van Zandvoort   +6 more
wiley   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Surface Roughness Improvement by Sliding Friction Burnishing of Parts Produced by Selective Laser Melting of Ti6Al4V Titanium Alloy

Machines, 2022
Selective laser melting is a frequently used, powder bed fusion additive manufacturing technology for producing metallic parts. However, appropriate surface quality cannot be achieved, so post-processing is often necessary.
Gyula Varga, Gergely Dezső, Ferenc Szigeti   +2 more
doaj   +1 more source

Power Density Distribution for Laser Additive Manufacturing (SLM): Potential, Fundamentals and Advanced Applications

Technologies, 2018
Problems with the laser additive manufacturing of metal parts related to its low efficiency are known to hamper its development and application.
Alexander S. Metel, Michael M. Stebulyanin, Sergey V. Fedorov, Anna A. Okunkova   +3 more
doaj   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Peripheral Neutrophil Activation and Extracellular Trap Formation in Amyotrophic Lateral Sclerosis

Annals of Clinical and Translational Neurology, EarlyView.
Markers of neutrophil activation are increased in plasma during ALS, and markers of NET formation associate with ALS survival. ABSTRACT Objectives Peripheral neutrophil levels in amyotrophic lateral sclerosis (ALS) inversely correlate with survival, suggesting a role for neutrophils in disease progression.
Lillia A. Baird, Haley McQuown, Jihyun Park, Samuel J. Teener, Ian F. Webber‐Davis, Andrew D. Carter, Dae‐Gyu Jang, Eva L. Feldman, Stephen A. Goutman, Benjamin J. Murdock   +9 more
wiley   +1 more source

The ultrahigh functional response of CuAlMnNb shape memory alloy by selective laser melting

Journal of Materials Research and Technology, 2022
In this study, the Cu-11.85Al-3.88Mn-1.6Nb (wt.%) shape memory alloy with low cost and a highly functional response was manufactured by selective laser melting(SLM).
Xiaoying Xi, Jiantao Zhang, Hao Tang, Yang Cao, Zhiyu Xiao   +4 more
doaj   +1 more source