Results 71 to 80 of about 969,721 (312)

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

open access: yesFEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee   +3 more
wiley   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

open access: yesFEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova   +2 more
wiley   +1 more source

Molecular characterization of covRS mutations in M1UK Streptococcus pyogenes

open access: yesFEBS Open Bio, EarlyView.
Group A Streptococcus (GAS) acquires covRS mutations driving a hypervirulent bacterial state, frequently associated with invasive disease‐like necrotizing fasciitis. We demonstrate that the newly emerged M1UK GAS lineage can also acquire these mutations.
Jarrad Pritchard   +12 more
wiley   +1 more source

Self-Assessment System and Tax Compliance in Nigeria

open access: yes, 2022
The study conceptually examined the effect of self-assessment system on tax compliance in Nigeria using extensive review of existing literatures. Procedural Justice Theory was adopted to guide the present study this is because the theory postulate that the existence of fairness in procedures may lead to fairness in outcome.
openaire   +2 more sources

Closing the communications loop on the computerized peer‐assessment of essays [PDF]

open access: yes, 2003
The use of self‐ and peer‐assessment is not new to higher education. Traditionally its use has required the complex and time‐consuming management of coursework submissions by the tutor, in an attempt to maintain validity and anonymity of the assessment ...
Davies, Phil, Phil Davies
core   +1 more source

From energy provision to protein synthesis: Tunnelling nanotubes as mediators of intercellular metabolic cooperation in cancer

open access: yesFEBS Open Bio, EarlyView.
The cytoskeleton‐mediated transport of mitochondria via tunnelling nanotubes restores respiration, increases ATP production, rescues cells from apoptosis, activates the AKT/mTOR signalling pathway, promotes cell migration and invasiveness, contributes to cancer progression and treatment resistance.
Stanislava Martínková, Jan Trnka
wiley   +1 more source

Competency self-assessment for a learning-based autonomous aircraft system

open access: yesFrontiers in Aerospace Engineering
IntroductionFuture concepts for airborne autonomy point toward human operators moving out of the cockpit and into supervisory roles. Urban air mobility, airborne package delivery, and military intelligence, surveillance, and reconnaissance (ISR) are all ...
Nicholas Conlon   +10 more
doaj   +1 more source

Self-Assessment and Self-Reflection to Measure and Improve Self-Regulated Learning in the Workplace

open access: yes, 2018
People’s self-monitoring of their learning has extensive impact on generating opportunities for professional development. Self-monitoring before, during, and after completing work-related tasks affects decision-making, learning behavior, strategy use ...
Mariëtte H. van Loon   +1 more
core   +1 more source

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