Results 321 to 330 of about 5,890,604 (383)

Cloning, expression, purification, and structural modeling of the Chandipura virus matrix protein

FEBS Open Bio, EarlyView.
This research protocol offers a guide for the cloning, expression, and purification of the Chandipura virus matrix protein using E. coli. It also includes a step‐by‐step procedure for cloning, expressing, and conducting fluorescence imaging of GFP‐fused Chandipura virus matrix protein in mammalian cell lines.
Mariana Grieben
wiley   +1 more source

PAM-readID is a rapid, simple, and accurate PAM determination method for CRISPR-Cas enzymes in mammalian cells. [PDF]

Commun Biol
Wang Q, Wang Z, Liu H, Lv Y, Zhou C, Li C, Fan H, Ouyang X, Tao L, Pei X, Xie T.   +10 more
europepmc   +1 more source

Homologous expression and purification of human HAX‐1 for structural studies

FEBS Open Bio, EarlyView.
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley   +1 more source

A single-copy knock-in system: one plasmid to target all chromosomes in C. elegans. [PDF]

G3 (Bethesda)
Dinneen E, Dasgupta P, Sharma A, Nisaa K, Silva-García CG.   +4 more
europepmc   +1 more source

The Role of AI-Driven De Novo Protein Design in the Exploration of the Protein Functional Universe. [PDF]

Biology (Basel)
Zhang G, Liu C, Lu J, Zhang S, Zhu L.
europepmc   +1 more source

Evaluating machine learning approaches for host prediction using H3 influenza genomic data. [PDF]

PLoS One
Tran H, Berke O, Ricker N, Poljak Z.
europepmc   +1 more source

Zero-shot segmentation using embeddings from a protein language model identifies functional regions in the human proteome. [PDF]

PLoS Comput Biol
Sangster AG, Dufault C, Qu H, Le D, Forman-Kay JD, Moses AM.   +5 more
europepmc   +1 more source

Heterologous expression of HCoV-HKU1 ORF 7b by mouse hepatitis virus protects against severe disease during murine infection. [PDF]

PLoS Pathog
Gunawardene CD, Pandey I, Chatterjee S, Peñaflor-Téllez Y, Odle A, Warren AN, Messyasz A, Rajsbaum R, Sariol A, Wong LR.   +9 more
europepmc   +1 more source

Exploring the genetic landscape of the Copper Homeostasis and Silver Resistance Island (CHASRI) in Salmonella enterica. [PDF]

PLoS One
Haendiges J, Brown EW, Ferreira C, Hoffmann M, Reed E, Zheng J, Tikekar R.   +6 more
europepmc   +1 more source

More biology from the sequence

, 2001
openaire   +1 more source