Results 121 to 130 of about 104,871 (266)

Characterization of a local case of severe fever with thrombocytopenia syndrome in Shenzhen, China: Clinical and immunological insights

Virus Research
Background: An exhaustive analysis of the immunological and pathogenic features of the first locally acquired case of severe fever with thrombocytopenia syndrome (SFTS) in Shenzhen was conducted. Methods: Serum samples were dynamically collected from one
Xiaomin Zhang, Jing Wu, Yalan Huang, Chunli Wu, Yue Li, Renli Zhang, Xiaohe Li, Bo Peng, Fan Yang   +8 more
doaj   +1 more source

Nonviral Gold Nanoparticle‐Mediated Delivery of CRISPR‐Cas9 Ribonucleoprotein and Long DNA Transgenes Into Primary Blood Cells

Advanced NanoBiomed Research, EarlyView.
CRISPR/Cas9 has revolutionized the field of gene therapy, but delivery remains an outstanding issue. We propose a nonviral gold‐nanoparticle platform for co‐delivery of CRISPR/Cas9 ribonucleoprotein and long 2.1 kilobase dsDNA transgene constructs. This CRISPR‐AuNP is inexpensive to produce and mediate gene editing and DNA delivery in T cells and CD34+
Rachel A. Cunningham, Karthikeya S. V. Gottimukkala, Daniel D. Lane, Katrina Poljakov, Patricia Lipson, Mark R. Enstrom, Alessandro Rizzi, Aude G. Chapuis, Jennifer E. Adair   +8 more
wiley   +1 more source

Characterization of the Lactococcus lactis Nisin A Operon Genes nisP, Encoding a Subtilisin-Like Serine Protease Involved in Precursor Processing, and nisR, Encoding a Regulatory Protein Involved in Nisin Biosynthesis [PDF]

, 1993
Biosynthesis of the lantibiotic peptide nisin by Lactococcus lactis NIZO R5 relies on the presence of the conjugative transposon Tn5276 in the chromosome. A 12-kb DNA fragment of Tn5276 including the nisA gene and about 10 kb of downstream DNA was cloned
Beerthuyzen, Marke M., Polman, Joyce,, Beerthuyzen, Marke M.,, Kuipers, Oscar P.,, Kuipers, Oscar P., Polman, Joyce, Siezen, Roland J., Vos, Willem M. de, Vos, Willem M. de,, Meer, Jan Roelof van der,, Siezen, Roland J.,   +10 more
core  

Targeting Amastigote and Trypomastigote Phases: Multi‐Epitope Vaccine Strategy Against Trypanosoma cruzi

Biotechnology and Applied Biochemistry, EarlyView.
ABSTRACT Effective vaccines against Trypanosoma cruzi, the causative agent of Chagas disease, are urgently needed. Here, we report the design and in silico validation of a novel multiepitope vaccine construct targeting the key surface proteins ASP‐2 and gp82. Using a comprehensive immunoinformatics pipeline, we identified and selected 38 potent T‐cell (
Maria Karolaynne da Silva, Caio Patrício de Souza Sena, Davi Serradella Vieira, Magdi E. A. Zaki, Leow Chiuan Yee, Shahina Akter, Umberto Laino Fulco, Jonas Ivan Nobre Oliveira, João Firmino Rodrigues‐Neto   +8 more
wiley   +1 more source

p23, a novel mammalian nucleic acid-binding protein with homology to the yeast ribosomal protein YL43

, 1992
When separating perchloric acid-soluble proteins from cell cultures and tissues by chromatography on single stranded DNA agarose columns, a novel mammalian protein with extreme affinity for DNA was isolated.
Anne Carine Østvold, Knut Sletten, Hullstein, Ingunn, Østvold, Anne Carine, Sletten, Knut, Ingunn Hullstein   +5 more
core   +1 more source

A Modular Nucleoside Kinase Cascade for the Synthesis of Ribonucleoside Triphosphates

Biotechnology and Bioengineering, EarlyView.
Damm et al. report standardized enzymatic cascades for production of all canonical nucleoside triphosphates from the corresponding nucleosides, using kinase‐catalyzed phosphorylation from acetyl phosphate. Reactions afforded ∼ 100 mM product in near‐quantitative conversion.
Oliver T. Damm, Martin Pfeiffer, Johannes Zöhrer, Bernd Nidetzky   +3 more
wiley   +1 more source

Human protein function prediction: application of machine learning for integration of heterogeneous data sources

, 2010
Experimental characterisation of protein cellular function can be prohibitively expensive and take years to complete. To address this problem, this thesis focuses on the development of computational approaches to predict function from sequence.
Lobley, A.E.
core  

Exploring the function and evolution of proteins using domain families [PDF]

, 2009
Proteins are frequently composed of multiple domains which fold independently. These are often evolutionarily distinct units which can be adapted and reused in other proteins.
Reid, A.J., Reid, A J
core  

RNA‐centric world of retroviruses: unravelling the molecular strategies of genomic RNA packaging

Biological Reviews, EarlyView.
ABSTRACT Retroviruses constitute a unique group of RNA viruses that have profoundly influenced both evolutionary trajectories and biomedical research. Their ability to reverse transcribe and integrate into host genomes has shaped genomic architecture across species and contributed to our understanding of oncogenes, gene regulation, and RNA biology ...
Mohammad Abdullah Jehad, Suresha G. Prabhu, Lizna M. Ali, Vineeta N. Pillai, Farah Mustafa, Tahir A. Rizvi   +5 more
wiley   +1 more source

The plasma protein fetuin: common structural features of the mammalian fetuin family

, 1991
The structure and tissue-specific expression of the bovine plasma glycoprotein fetuin and its homologues in other species has been examined. From the known partial amino acid sequence of bovine fetuin, degenerate oligonucleotide probes were designed ...
Brown, William Michael
core