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A gene cluster for the synthesis of serotype d-specific polysaccharide antigen in Actinobacillus actinomycetemcomitans

Biochimica Et Biophysica Acta Gene Regulatory Mechanisms, 2000
The serotype d antigen of Actinobacillus actinomycetemcomitans consists of D-glucose, D-mannose, and L-rhamnose in a molar ratio of 1:2:1. A gene cluster involved in the synthesis of serotype-specific polysaccharide antigen was cloned from the chromosomal DNA of A. actinomycetemcomitans IDH 781 (serotype d).
Yoshio J Nakano
exaly   +3 more sources

Identification and Detection of Serotype-Specific Genes: Effective Serotyping of Streptococcus suis

Current Clinical Microbiology Reports, 2017
Streptococcus suis is an emerging zoonotic pathogen that can cause severe infections in pigs and humans. S. suis has been reported in Europe, America, and Asia, and has resulted in heavy losses to swine production and serious public health concerns. Serum agglutination has identified several serotypes. Here, we review the methods for serotyping S. suis,
Kaicheng Wang   +4 more
openaire   +1 more source

Genetic analysis of the gene cluster for the synthesis of serotype a-specific polysaccharide antigen in Actinobacillus actinomycetemcomitans

Biochimica Et Biophysica Acta Gene Regulatory Mechanisms, 2000
The serotype a-specific polysaccharide antigen (SPA) of Actinobacillus actinomycetemcomitans consists of 6-deoxy-D-talose. A gene cluster associated with the biosynthesis of SPA was cloned and sequenced from the chromosomal DNA of A. actinomycetemcomitans SUNYaB 75 (serotype a). This cluster consisted of 14 open reading frames. Insertional inactivation
Yoshio J Nakano
exaly   +3 more sources

Sequence analysis of the gene encoding the serotype-specific glycoprotein (VP7) of two new human rotavirus serotypes

Virology, 1989
Human rotavirus strains 69M and WI61 are distinct from human rotavirus serotypes 1, 2, 3, and 4 and from each other by plaque reduction neutralization and have been proposed as new human rotavirus serotypes (serotype 8 and serotype 9, respectively). The nucleotide sequence of the gene encoding the serotype-specific capsid glycoprotein, VP7, of strains ...
K Y, Green, Y, Hoshino, N, Ikegami
openaire   +2 more sources

Genetic analysis of the gene cluster responsible for synthesis of serotype e-specific polysaccharide antigen in Actinobacillus actinomycetemcomitans

Biochimica Et Biophysica Acta Gene Regulatory Mechanisms, 1999
A gene cluster associated with the biosynthesis of the serotype e-specific polysaccharide antigen (SPA) of Actinobacillus actinomycetemcomitans IDH1705 belonging to serotype e was cloned and sequenced. This cluster consisted of 18 open reading frames. Escherichia coli produced the polysaccharide that reacts with the serotype e-specific antiserum when ...
Yoshio J Nakano
exaly   +3 more sources

Molecular analysis of the genes involved in the biosynthesis of serotype specific polysaccharide in the novel serotype k strains of Streptococcus mutans

Oral Microbiology and Immunology, 2005
We previously reported the new serotype k of Streptococcus mutans, which, compared to serotypes c, e, and f, features a drastic reduction in the length of the glucose side chain linked to the rhamnose backbone of the serotype specific polysaccharide. The 5′ region of the rgpF gene of serotype k strains contains a distinctive nucleotide sequence, which ...
R, Nomura, K, Nakano, T, Ooshima
openaire   +2 more sources

The O-specific polysaccharide structure and biosynthetic gene cluster of Yersinia pseudotuberculosis serotype O:11

Carbohydrate Research, 2009
In the Yersinia pseudotuberculosis serotyping scheme, 21 serotypes are present originating from about 30 different O-factors distributed within the species. With regard to the chemical structures of lipopolysaccharides (LPSs) and the genetic basis of their biosynthesis, a number, but not all, of Y.
M. M. Cunneen   +7 more
openaire   +4 more sources

Selection of serotype-specific vaccine candidate genes in Actinobacillus pleuropneumoniae and heterologous immunization with Propionibacterium acnes

Vaccine, 2008
Actinobacillus pleuropneumoniae (A. pleuropneumoniae) is a highly contagious lethal causative agent of swine pleuropneumoniae. Vaccines for this disease are usually serotype specific. In order to identify immunogenic genes specific to serotypes, two differentially expressed gene cDNA libraries of A.
Liancheng, Lei   +5 more
openaire   +2 more sources

Molecular Characterization of a Gene Locus Encoding Biosynthesis Pathway of Streptococcus mutans Serotype C-Specific Antigen

1997
Almost all streptococci possess rhamnose in their cell-wall polysaccharides as a characteristic constituent. Many streptococcal cell-wall polysaccharides have been chemically characterized and it was found that serotype-specific polysaccharide antigens of Streptococcus mutans are composed of polyrhamnose backbone structure identical to those of the ...
Y, Yamashita   +4 more
openaire   +2 more sources

Cloning, sequencing and expression of the gene that encodes the major neutralisation-specific antigen of African horsesickness virus serotype 9

Journal of Virological Methods, 2000
A marked improvement in the efficiency of cloning the large double stranded RNA (dsRNA) genome segments of African horsesickness virus (AHSV) was achieved when the dsRNA polyadenylation step was carried out with undenatured rather than strand-separated dsRNA.
M, Venter, G, Napier, H, Huismans
openaire   +2 more sources

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