Results 71 to 80 of about 1,286,865 (349)

Phosphomimetic mutations near active sites of proteins in Thermus thermophilus suggest a widespread regulatory mechanism

FEBS Open Bio, EarlyView.
In Thermus thermophilus, more than half of the phosphorylation sites identified by proteomic analysis are located near the active site. All phosphomimetic mutants of phosphosites of six enzymes belonging to different families showed severely reduced activity compared with the wild‐type, particularly in the turnover number.
Anzu Nishiwaki, Hiroki Okanishi, Yoshikatsu Kanai, Ryoji Masui   +3 more
wiley   +1 more source

Ultrasensitive SERS-Based Plasmonic Sensor with Analyte Enrichment System Produced by Direct Laser Writing

Nanomaterials, 2019
We report an easy-to-implement device for surface-enhanced Raman scattering (SERS)-based detection of various analytes dissolved in water droplets at trace concentrations. The device combines an analyte-enrichment system and SERS-active sensor site, both
Georgii Pavliuk, Dmitrii Pavlov, Eugeny Mitsai, Oleg Vitrik, Aleksandr Mironenko, Alexander Zakharenko, Sergei A. Kulinich, Saulius Juodkazis, Svetlana Bratskaya, Alexey Zhizhchenko, Aleksandr Kuchmizhak   +10 more
doaj   +1 more source

A method for benchmarking two different resilience assessment methods

Ecology and Society, 2022
Assessments of objective and subjective resilience are the main methods for understanding disaster preparedness and developing policy responses. However, the two assessment outcomes are not directly comparable.
Song Yong, Yiheyis T Maru, Alexander Herr, Thomas G Measham, Barton Loechel   +4 more
doaj   +1 more source

Surface Enhanced Raman Scattering of p-(Dimethylamino) Cinnamic Acid on Silver and Silver-Gold Alloids [PDF]

, 2014
Through this experimental work we describe assignments for the Raman and surface-enhanced Raman scattering (SERS) spectra (on Ag and Ag(x)-Au(1-x) colloids) of 4-(dimethylamino) cinnamic acid (DMACA).
Tanriver, Ezgi
core   +1 more source

Rethinking plastic waste: innovations in enzymatic breakdown of oil‐based polyesters and bioplastics

FEBS Open Bio, EarlyView.
Plastic pollution remains a critical environmental challenge, and current mechanical and chemical recycling methods are insufficient to achieve a fully circular economy. This review highlights recent breakthroughs in the enzymatic depolymerization of both oil‐derived polyesters and bioplastics, including high‐throughput protein engineering, de novo ...
Elena Rosini, Nicolò Antonelli, Gianluca Molla   +2 more
wiley   +1 more source

Surfactant-free solution-based synthesis of metallic nanoparticles toward efficient use of the nanoparticles’ surfaces and their application in catalysis and chemo-/biosensing

Nanotechnology Reviews, 2013
The choice of stabilizer and the stabilizer-to-precursor ions molar ratio during metal nanoparticle synthesis are important for controlling the shape, size, and dispersion stability of the nanoparticles.
Kawasaki Hideya
doaj   +1 more source

Synthesis of Monolayer Gold Nanorings Sandwich Film and Its Higher Surface-Enhanced Raman Scattering Intensity

Nanomaterials, 2020
Most previous studies relating to surface-enhanced Raman spectroscopy (SERS) signal enhancement were focused on the interaction between the light and the substrate in the x-y axis.
Liqiu Zhang, Tiying Zhu, Cheng Yang, Ho Young Jang, Hee-Jeong Jang, Lichun Liu, Sungho Park   +6 more
doaj   +1 more source

Examination of Silica Sol-Gels and Aerogels Containing Silver Nanoparticles and 4-Mercaptobenzoic Acid Using Surface-Enhanced Raman Spectroscopy [PDF]

, 2016
Sol-gels and aero-gels containing silver nanoparticles have been investigated for use as substrates in surface-enhanced Raman spectroscopy (SERS).
Atkinson, Elizabeth J.O., Corrado, Tanner, Gilbert, Brian D.   +2 more
core   +1 more source

Cloning, expression, purification, and structural modeling of the Chandipura virus matrix protein

FEBS Open Bio, EarlyView.
This research protocol offers a guide for the cloning, expression, and purification of the Chandipura virus matrix protein using E. coli. It also includes a step‐by‐step procedure for cloning, expressing, and conducting fluorescence imaging of GFP‐fused Chandipura virus matrix protein in mammalian cell lines.
Mariana Grieben
wiley   +1 more source

Homologous expression and purification of human HAX‐1 for structural studies

FEBS Open Bio, EarlyView.
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley   +1 more source