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Generation of Advanced Blood–Brain Barrier Spheroids Using Human‐Induced Pluripotent Stem Cell‐Derived Brain Capillary Endothelial‐Like Cells

open access: yesAdvanced Biology, Volume 9, Issue 4, April 2025.
In this article, the establishment and characterization of a self‐assembled 3D BBB spheroid model using human induced pluripotent stem cell (hiPSC)‐derived and primary cells is reported. Spheroids demonstrate in‐vivo like tight junction ultrastructure and, in comparison to 2D mono‐cultures, higher transcript expression of BBB specific genes.
Sanjana Mathew‐Schmitt   +10 more
wiley   +1 more source

Optimizing Liquidity Usage and Settlement Speed in Payment Systems [PDF]

open access: yes
The operating speed of a payment system depends on the stage of technology of the system's communication and information processing environment. Frequent intraday processing cycles and real-time processing have introduced new means of speeding up the ...
Leinonen, Harry, Soramäki, Kimmo
core  

Characterization and Optimization of Vesicle Properties in bioPISA: from Size Distribution to Post‐Assembly Loading

open access: yesAdvanced Biology, Volume 9, Issue 5, May 2025.
The paper explores the creation and characterization of vesicles through biocatalytic Polymerization‐Induced Self‐Assembly (bioPISA), focusing on achieving size uniformity using centrifugation techniques. It examines the effects of stirring speed on vesicle morphology and analyses the internal polymer‐rich structure using fluorescence correlation ...
Andrea Belluati   +7 more
wiley   +1 more source

Depletion of TP53 in Human Pluripotent Stem Cells Triggers Malignant‐Like Behavior

open access: yesAdvanced Biology, Volume 9, Issue 4, April 2025.
Human pluripotent stem cells (hPSCs) with TP53‐depleting mutations are generated, mimicking commonly occurring events in vitro. Loss of TP53 does not alter pluripotency status or in vitro differentiation potential but causes genetic instability and transcriptome changes and confers superior clonal and anchorage‐free growth and higher chemotherapeutic ...
Joaquin Montilla‐Rojo   +9 more
wiley   +1 more source

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