Results 71 to 80 of about 19,239 (237)

Monitoring and stability analysis of roadbed & high slope prior to highway construction.

open access: yesPLoS ONE
Monitoring in highway projects is significant for the safety, efficiency and quality of construction. This paper proposes a detailed method for monitoring soft-soil roadbed and high-slope, and the layered settlement gauge and total station are employed ...
Rengye Zhao   +3 more
doaj   +1 more source

In silico and in vitro exploration of a tyrosinase for biocatalytic production of catechols

open access: yesFEBS Open Bio, EarlyView.
Tyrosinase from Ralstonia pseudosolanacearum is a promising biocatalyst for producing valuable catechols from monophenol substrates. This tyrosinase is uniquely suited to this due to its high monophenolase : diphenolase ratio. We combined in silico docking and in vivo kinetic characterisation of this tyrosinase with 11 industrially relevant monophenols,
James Britton   +6 more
wiley   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

open access: yesFEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen   +5 more
wiley   +1 more source

Avidin is evolutionarily conserved in fish but dispensable for development and resistance against Streptococcus agalactiae in zebrafish

open access: yesFEBS Open Bio, EarlyView.
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti   +5 more
wiley   +1 more source

Early warning of settlement and deformation of high-rise buildings on soft foundations in karst areas

open access: yesCarsologica Sinica
The background of this article pertains to a high-rise building project, which encompasses a building land area of about 4,630 m2 and features a roughly square shape in its plane. The safety classification of the building structure is designated as Level
Lin MA
doaj   +1 more source

GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma

open access: yesFEBS Open Bio, EarlyView.
GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain   +11 more
wiley   +1 more source

Prediction and Engineering Verification of Building Settlement in Loess High-Fill Areas

open access: yesBuildings
To identify suitable settlement prediction methods for buildings constructed in loess high-fill areas, this study analyzes settlement monitoring data from a residential building in the Yan’an New District.
Zhipeng Huo, Xukun Han, Yafei He
doaj   +1 more source

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