Results 131 to 140 of about 68,716 (153)

A novel SGNH family hydrolase Ali5 with thioesterase activity and a GNSL motif but without a classic GDSL motif from Altererythrobacter ishigakiensis

Biotechnology Letters, 2019
We aimed to characterize a novel SGNH (Ser-Gly-Asn-His) family hydrolase from the annotated genome of marine bacteria with new features.A novel esterase Ali5 from Altererythrobacter ishigakiensis has been identified and classified into SGNH family. Ali5 presented a novel GNSL (Gly-Asn-Ser-Leu(X)) motif that differs from the classic GDSL (Gly-Asp-Ser ...
Xiao-Jian Hu, Jixi Li, Heng-Lin Cui
exaly   +4 more sources

Crystal Structure of Escherichia coli Thioesterase I/Protease I/Lysophospholipase L1: Consensus Sequence Blocks Constitute the Catalytic Center of SGNH-hydrolases through a Conserved Hydrogen Bond Network

Journal of Molecular Biology, 2003
Escherichia coli thioesterase I (TAP) is a multifunctional enzyme possessing activities of thioesterase, esterase, arylesterase, protease, and lysophospholipase. In particular, TAP has stereoselectivity for amino acid derivative substrates, hence it is useful for the kinetic resolution of racemic mixtures of industrial chemicals.
Su-Chang Lin
exaly   +4 more sources

Characterization of a Highly Solvent-Tolerant SGNH Hydrolase Superfamily Lipolytic Enzyme from Sphaerobacter thermophilus.

Biochemistry
Thermophilic microbial lipases that retain activity under harsh conditions are a highly desirable tool for catalysis in numerous biosynthetic and biotechnological applications.
Kelsey Minium, Zachary J. Knepp, Morgan Sutton, Tabatha Falls, Sara Bobb, Connor McKeefery, Kailynn Smith, Kyle T Root   +7 more
semanticscholar   +3 more sources

Identification and Characterization of a New Alkaline SGNH Hydrolase from a Thermophilic Bacterium Bacillus sp. K91.

Journal of Microbiology and Biotechnology, 2016
est19 is a gene from Bacillus sp. K91 that encodes a new esterase. A comparison of the amino acid sequence showed that Est19 has typical Ser-Gly-Asn-His (SGNH) family motifs and could be grouped into the SGNH hydrolase family. The Est19 protein was functionally cloned, and expressed and purified from Escherichia coli BL21(DE3).
Tingting Yu, Junmei Ding, Q. Zheng, Nanyu Han, Jialin Yu, Yunjuan Yang, Junjun Li, Yuelin Mu, Qian Wu, Zunxi Huang   +9 more
semanticscholar   +3 more sources

Rhamnogalacturonan Acetylesterase, a Member of the SGNH-Hydrolase Family

, 2003
Rhamnogalacturonan acetylesterase (RGAE) from Aspergillus aculeatus acts in synergy with rhamnogalacturonases on the enzymatic degradation of rhamnogalacturonan I (RG-I). RGAE is a member of the Carbohydrate Esterase Family 12 (CEF 12), which also includes two pectin acetylesterases, a cephalosporin C deacetylase and a number of bacterial proteins of ...
A. Mølgaard
semanticscholar   +2 more sources

Catalytic Dyad in the SGNH Hydrolase Superfamily: In-depth Insight into Structural Parameters Tuning the Catalytic Process of Extracellular Lipase from Streptomyces rimosus.

ACS Chemical Biology, 2017
SrLip is an extracellular enzyme from Streptomyces rimosus (Q93MW7) exhibiting lipase, phospholipase, esterase, thioesterase, and tweenase activities. The structure of SrLip is one of a very few lipases, among the 3D-structures of the SGNH superfamily of hydrolases, structurally characterized by synchrotron diffraction data at 1.75 Å resolution (PDB ...
Ivana Leščić Ašler, Z. Štefanić, A. Maršavelski, R. Vianello, B. Kojić-Prodić   +4 more
semanticscholar   +5 more sources

Probing Enzyme Promiscuity of SGNH Hydrolases

ChemBioChem, 2010
AbstractSeveral hydrolases of the SGNH superfamily, including the lipase SrLip from Streptomyces rimosus (Q93MW7), the acyl‐CoA thioesterase I TesA from Pseudomonas aeruginosa (Q9HZY8) and the two lipolytic enzymes EstA (from P. aeruginosa, O33407) and EstP (from Pseudomonas putida, Q88QS0), were examined for promiscuity.
Leščić Ašler, Ivana, Ivić, Nives, Kovačić, Filip, Schell, Sabrina, Knorr, Janina, Krauss, Ulrich, Wilhelm, Susanne, Kojić-Prodić, Biserka, Jaeger, Karl-Erich   +8 more
openaire   +2 more sources

Heterologe Expression und Charakterisierung einer GDS(L)‐ähnlichen Hydrolase aus Pleurotus sapidus mit einem ungewöhnlichen SGNH‐Motiv im aktiven Zentrum

Lebensmittelchemie
M. Fingerhut, L. Henrich, C. Lauber, N. Broel, P. Ghezellou, D. Karrer, B. Spengler, K. Langfelder, T. Stressler, H. Zorn, M. Gand   +10 more
semanticscholar   +2 more sources

How promiscuous are SGNH-hydrolases?

2008
Although lipolytic enzymes are known for decades, the classification of this type of enzymes is a continuous process. Among many defined classes and families, a new one was recently identified: SGNH-hydrolases (1). According to InterPro database (March 2008.), there are 3775 members of this family, with protein sequences originating mainly from genome ...
Kojić-Prodić, Biserka, Ivić, Nives, Leščić Ašler, Ivana   +2 more
openaire   +3 more sources

The SGNH-hydrolases from streptomycetes

2009
Lipases (triacylglycerol acylhydrolases, EC 3.1.1.3) are hydrolytic enzymes widely distributed in microorganisms, plants and animals. Microbial lipases from a wide range of species have been subject of many important studies in the past few years because of the large number of reactions they catalyze.
Vujaklija, Dušica, Pigac, Jasenka, Abramić, Marija, Bielen, Ana   +3 more
openaire   +1 more source