FEBS Open Bio, EarlyView.CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point +7 more
wiley +1 more source
Shaking Table Model Test and Seismic Performance Analysis of a High-Rise RC Shear Wall Structure
Shock and Vibration, 2019 A building developed by Wuhan Shimao Group in Wuhan, China, is a high-rise residence with 56 stories near the Yangtze River. The building is a reinforced concrete structure, featuring with a nonregular T-type plane and a height 179.6 m, which is out of ...
Shujin Li, Cai Wu, Fan Kong
doaj +1 more source
Study of new rare event simulation schemes and their application to extreme scenario generation [PDF]
, 2018 This is a companion paper based on our previous work on rare event simulation methods. In this paper, we provide an alternative proof for the ergodicity of shaking transformation in the Gaussian case and propose two variants of the existing methods ...
Agarwal, Ankush +3 more
core +1 more source
Alcohol‐induced altered glycans in human tracheal epithelial cells promote bacterial adhesion
FEBS Open Bio, EarlyView.Alcohol induces altered glycans to promote bacteria adhesion. Heavy alcohol drinking is known to increase the risk of bacterial pneumonia. However, the link between alcohol levels and risk of infection remains underexplored. Recently, we found that alcohol induced α2‐6sialo mucin O‐glycans in human tracheobronchial epithelial cells, which mediated the ...
Pi‐Wan Cheng +2 more
wiley +1 more source
Modified Gutenberg-Richter coefficient for damage evaluation in reinforced concrete structures subjected to seismic simulations on a shaking table [PDF]
, 2014 This paper presents analysis and discussion of the b- and ib-values calculated from the acoustic emission (AE) signals recorded during dynamic shake-table tests conducted on a reinforced concrete (RC) frame subjected to several uniaxial seismic ...
Benavent Climent, Amadeo +3 more
core +2 more sources
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
FEBS Open Bio, EarlyView.Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
Application of the Modified Mohr–Coulomb Yield Criterion in Seismic Numerical Simulation of Tunnels
Shock and Vibration, 2021 To solve the classical problem that the Mohr–Coulomb yield criterion overestimates the tensile properties of geotechnical materials, a modified Mohr–Coulomb yield criterion that includes both maximum tensile stress theory and smooth processing was ...
Chuan-Yi Sui +3 more
doaj +1 more source
Shaking table tests of stone masonry buildings [PDF]
, 2010 This paper presents the main results of experimental tests concerning the reduction of the seismic vulnerability of stone masonry buildings with flexible floors.
Costa, Alfredo Campos +2 more
core
Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function
FEBS Open Bio, EarlyView.We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke +4 more
wiley +1 more source
Accuracy Assessment for the Three-Dimensional Coordinates by High-Speed Videogrammetric Measurement
Journal of Electrical and Computer Engineering, 2018 High-speed CMOS camera is a new kind of transducer to make the videogrammetric measurement for monitoring the displacement of high-speed shaking table structure.
Xianglei Liu, Yi Tang, Jing Ma
doaj +1 more source