Results 51 to 60 of about 2,218,481 (259)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source

Transcripts enriched in codons that trigger P‐site tRNA‐mediated mRNA decay possess stable mRNA

FEBS Open Bio, EarlyView.
PTMD codons were first described by Mendel et al. as mediators of an mRNA decay pathway dependent on the human protein CNOT3, homologous to yeast Not5. Our findings confirm that PTMD codons destabilize transcripts; however, unlike in yeast, the human pathway specifically targets and slightly destabilizes primarily stable mRNAs.
Rodolfo Lopes Carneiro, Fernando Lucas Palhano   +1 more
wiley   +1 more source

Adaptive finite element simulation of sheet forming process parameters

Journal of King Saud University: Engineering Sciences, 2018
The forming processes are influenced by several parameters including dimensions of blank, shape of the tools, mechanical properties of the blank material and type of forming process.
Mohd. Ahmed
doaj   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Structural and biochemical insights into the thermostable esterase Ta0887 from Thermoplasma acidophilum

FEBS Open Bio, EarlyView.
In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey, Miriam Livier Llamas‐García, Gabriela M. Montero‐Morán, Leticia Santos, Samuel Lara‐González   +4 more
wiley   +1 more source

Effect of stress relaxation on the sealing performance of O-rings in deep-sea hydraulic systems: A numerical investigation

Engineering Science and Technology, an International Journal
A reliable O-ring seal is an important guarantee for the stable running of deep-sea hydraulic systems and for the execution and completion of subsea tasks.
Jia-Bin Wu, Li Li, Pin-Jian Wang
doaj   +1 more source

Analysis and Design of Reflectarray Antennas Based on Delay Lines: A Filter Perspective

IEEE Access, 2020
The analysis and design of reflectarray (RA) antennas based in delay lines is introduced for the first time from a filter perspective. To this purpose, each unit-cell of the RA is considered as a network composed of two ports, one being the delay line ...
Jiawei Zang, Eduardo Carrasco, Xuetian Wang, Alejandro Alvarez-Melcon, Juan Sebastian Gomez-Diaz   +4 more
doaj   +1 more source

BCG vaccination potentiates oxidative phosphorylation in neonatal myeloid‐derived suppressor cells

FEBS Open Bio, EarlyView.
BCG vaccination enhances oxidative phosphorylation in neonatal MDSCs, impairing their immunosuppressive function. It upregulates electron transport chain genes and mitochondrial activity, increasing ATP and oxygen consumption. Pharmacological OXPHOS inhibition partially restores suppressive capacity, confirming causality.
Yingying Chen, Hui Li
wiley   +1 more source

Simulation and test of a V-shaped seed-guiding surface and concave pit furrow opener for seed displacement restriction: Verification on seeding uniformity and post-soil-contact seed motion limitation.

PLoS ONE
In plot breeding tests, sowing uniformity directly impacts the accuracy of crop genetic improvement trials. Existing furrow openers often cause spacing fluctuations and lateral seed drift due to structural limitations.
Guoqiang Dun, Yang He, Xinxin Ji, Chunyu Ma, Xinming Li, Luhan Wang   +5 more
doaj   +1 more source