Results 41 to 50 of about 133,408 (262)

Homologous expression and purification of human HAX‐1 for structural studies

FEBS Open Bio, EarlyView.
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley   +1 more source

Probing the Synthesis of Two-Dimensional Boron by First-Principles Computations

, 2013
Boron synthesis, in theory: Although two-dimensional boron sheets have attracted considerable interest because of their theoretically predicted properties, synthesis of such sheets remains a challenge.
Liu, Yuanyue, Penev, Evgeni S., Yakobson, Boris I.   +2 more
core   +1 more source

SmallTalk: a novel small‐sized fusion tag for peptide expression and purification

FEBS Open Bio, EarlyView.
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq, Nestor G. Casillas‐Vega, Alma Gomez‐Loredo, Xristo Zarate   +3 more
wiley   +1 more source

Enhancing Corrosion Resistance and Mechanical Strength of 3D‐Printed Iron Polylactic Acid for Marine Applications via Laser Surface Texturing

Advanced Engineering Materials, EarlyView.
Laser surface texturing significantly improves the corrosion resistance and mechanical strength of 3D‐printed iron polylactic acid (Ir‐PLA) for marine applications. Optimal laser parameters reduce corrosion by 80% and enhance tensile strength by 25% and ductility by 15%.
Mohammad Rezayat, Mojtaba Karamimoghadam, Mohammadreza Lalegani Dezaki, Ali Zolfagharian, Giuseppe Casalino, Antonio Mateo, Mahdi Bodaghi   +6 more
wiley   +1 more source

Study on Rotary Bending Forming Process of Torsional Damper Shell Pulley

Xibei Gongye Daxue Xuebao, 2019
To study the integral forming process of torsion damper shell pulley with characteristic structure is of great significance for producing and developing of the rotational part such as shell pulley. A processing way was proposed by combining plate bending

doaj   +1 more source

Progressive microforming of pin-shaped plunger parts and the grain size effect on its forming quality

Materials & Design, 2020
Progressive microforming of bulk microparts by directly using sheet metals has been developed to address the issues of preform/billet positioning, transportation and ejection in microforming, and it has potential to be used for making complex pin-shaped ...
Jun-Yuan Zheng, S.Q. Shi, M.W. Fu
doaj   +1 more source

Stretching Micro Metal Particles into Uniformly Dispersed and Sized Nanoparticles in Polymer. [PDF]

, 2017
There is a longstanding challenge to disperse metal nanoparticles uniformly in bulk polymers for widespread applications. Conventional scale-down techniques often are only able to shrink larger elements (such as microparticles and microfibers) into micro/
Cao, Chezheng, Hwang, Injoo, Javadi, Abdolreza, Li, Xiaochun, Lin, Ting Chang, Pozuelo, Marta, Yang, Yingchao, Zhao, Jingzhou   +7 more
core   +1 more source

Evaluation of the Potential of Laser Shock Peening Compared with Cold Expansion for Improving Fatigue Resistance of Riveted Lap Joints of Aerospace Grade 7175 Al Alloy

Advanced Engineering Materials, EarlyView.
This study investigates laser shock peening for enhancing fatigue performance of riveted aerospace aluminum joints. A comparative approach with cold expansion combines fatigue testing and synchrotron X‐ray methods. Integrating mechanical testing with residual stress and strain characterization provides insights into how different treatments affect the ...
Ogün Baris Tapar, David Osman Busse, Maximilian Sprengel, Domenico Furfari, Marc‐André Nielsen, Guilherme Abreu Faria, Jérémy Epp   +6 more
wiley   +1 more source

Selective oxidation of tool steel surfaces under a protective gas atmosphere using inductive heat treatment

MATEC Web of Conferences, 2018
For the realization of liquid lubricant free forming processes different approaches are conceivable. The priority program 1676 “Dry forming - Sustainable production through dry machining in metal forming” addresses this issue in the context of metal ...
Schöler S., Yilkiran D., Wulff D., Özkaya F., Möhwald K., Behrens B.-A., Maier H.J.   +6 more
doaj   +1 more source

On the Impact Energy Absorption of an Advanced High‐Strength Bilayer Steel Composite Consisting of Carbide‐Free Bainitic Steel and High‐Mn Twinning‐Induced Plasticity Steel

Advanced Engineering Materials, EarlyView.
Excellent combinations of strength and impact toughness are obtained in a bilayer composite of high‐strength carbide‐free bainitic (CFB) steel and high‐ductility twinning‐induced plasticity (TWIP) steel. The composite absorbs energy at small strains when the notch is placed on the TWIP side and at high strains when the notch is placed on the CFB side ...
Kendra Hawke, Xiaogang Li, Amir Partovi, Colin Scott, Fateh Fazeli, Hatem Zurob   +5 more
wiley   +1 more source