Results 41 to 50 of about 4,756,808 (297)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Obrazy Holocaustu w etiudach filmowych PWSFTviT w Łodzi

Images, 2011
In Serach of the Form. Images of the Holocaust in student films of the Polish National Film School   The article In Search of the Form is a contribution to the research on school films about Holocaust.
Katarzyna Mąka-Malatyńska
doaj   +1 more source

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

FEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya, Masatake Osawa, Hidefumi Fukumitsu   +2 more
wiley   +1 more source

Understanding Dutch film culture: a comparative approach [PDF]

, 2013
In terms of cinema attendance, the Netherlands has always differed from other European countries. During the first decade of permanent film exhibition—a crucial phase in cinema’s development as a mass medium—the movies failed to gain a firm foothold in ...
Thissen, Judith, Thissen, J., LS Film televisiegeschiedenis, Judith Thissen, ICON - Media and Performance Studies   +4 more
core   +2 more sources

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

FEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty, Kelsi R. Hall, Zachary D. Tillett, Liam S. Turk, James S. Davies, Michael J. Currie, Hamish G. Brown, Sepideh Valimehr, Peter D. Mace, Andrew E. Whitten, Santosh Panjikar, Eric Hanssen, Rachel A. North, Renwick C.J. Dobson   +13 more
wiley   +1 more source

Learning with Short Films: An Artistic Approach to Internal Medicine Nursing Education

Educational Academic Research
Nursing education is a comprehensive journey of development that goes far beyond technical skills, blending the student's ethical values and therapeutic communication skills with their professional identity. Innovative teaching models adopted during this
Amine Terzi
doaj   +1 more source

Un art du raccourci : La Ricotta de Pier Paolo Pasolini

Mise au Point, 2018
La Ricotta is short film of Pier Paolo Pasolini that creates many links, through the recreation of living paintings or in a more allusive way, with paintings developping an art of the foreshortening. As a twist, this foreshortening unfolds on the visual,
Cécile Sorin
doaj   +1 more source

De novo synthesis of short‐chain aldehydes and hydrocarbons secreted by the brown marmorated stink bug Halyomorpha halys

FEBS Open Bio, EarlyView.
The brown marmorated stink bug, Halyomorpha halys, mainly secretes 4‐oxo‐(E)‐2‐hexenal, (E)‐2‐decenal, dodecane and tridecane, a mixture representative of that released by many heteropterans. We demonstrated that H. halys can synthesise these secretory components de novo using dietary glucose.
Haruna Fujimori, Koji Noge
wiley   +1 more source

Imaginaire du scenario de court métrage

Mise au Point, 2018
Our aim is to identify the specificities of short film scripts, which are not limited to formal structures; this is why we suggest in this paper examining the imaginary constructs that determines both the possibility for the short film to exist and the ...
Sophie Beauparlant
doaj   +1 more source

Genetic dissection of human ABCE1 in yeast reveals separable requirements for ribosome recycling and suppression of aberrant reinitiation

FEBS Open Bio, EarlyView.
Human ABCE1 cannot functionally replace its yeast ortholog. Yeast–human chimera analysis identified NBD1 as a major interspecies barrier. Genetic screening yielded hABCE1 revertants that rescue yeast viability but fail to suppress aberrant translation reinitiation in the 3′ UTR.
Eriko Nakata, Yuxin Li, Kei Endo, Koichi Ito   +3 more
wiley   +1 more source