Results 31 to 40 of about 1,815,787 (311)

A Cre‐dependent lentiviral vector for neuron subtype‐specific expression of large proteins

open access: yesFEBS Letters, EarlyView.
We designed a versatile and modular lentivector comprising a Cre‐dependent switch and self‐cleaving 2A peptide and tested it for co‐expression of GFP and a 2.8 kb gene of interest (GOI) in mouse cortical parvalbumin (PV+) interneurons and midbrain dopamine (TH+) neurons.
Weixuan Xue   +6 more
wiley   +1 more source

Joint Use of e.Photoexpression© and Photonarration: What Methodological Added Value?

open access: yesFrontiers in Public Health, 2021
Objectives: The objective is to describe the informative value and the added value of a qualitative multiphase methodology in order to investigate the conceptions of children aged 6–11 on the determinants of health and cancer.Method: This article ...
Deyra Maéliane   +4 more
doaj   +1 more source

The Caenorhabditis elegans DPF‐3 and human DPP4 have tripeptidyl peptidase activity

open access: yesFEBS Letters, EarlyView.
The dipeptidyl peptidase IV (DPPIV) family comprises serine proteases classically defined by their ability to remove dipeptides from the N‐termini of substrates, a feature that gave the family its name. Here, we report the discovery of a previously unrecognized tripeptidyl peptidase activity in DPPIV family members from two different species.
Aditya Trivedi, Rajani Kanth Gudipati
wiley   +1 more source

Crosstalk between the ribosome quality control‐associated E3 ubiquitin ligases LTN1 and RNF10

open access: yesFEBS Letters, EarlyView.
Loss of the E3 ligase LTN1, the ubiquitin‐like modifier UFM1, or the deubiquitinating enzyme UFSP2 disrupts endoplasmic reticulum–ribosome quality control (ER‐RQC), a pathway that removes stalled ribosomes and faulty proteins. This disruption may trigger a compensatory response to ER‐RQC defects, including increased expression of the E3 ligase RNF10 ...
Yuxi Huang   +8 more
wiley   +1 more source

Real‐time assay of ribonucleotide reductase activity with a fluorescent RNA aptamer

open access: yesFEBS Letters, EarlyView.
Ribonucleotide reductases (RNR) synthesize DNA building blocks de novo, making them crucial in DNA replication and drug targeting. FLARE introduces the first single‐tube real‐time coupled RNR assay, which enables isothermal tracking of RNR activity at nanomolar enzyme levels and allows the reconstruction of allosteric regulatory patterns and rapid ...
Jacopo De Capitani   +4 more
wiley   +1 more source

Simp czy sigma? Wizerunek Stanisława Wokulskiego w memach

open access: yesLiteratura i Kultura Popularna
This article discusses the image of Stanisław Wokulski presented in selected memes and determines its connections with the present-day cultural imaginary regarding Bolesław Prus’s novel Lalka [The Doll].
Anna Lauks
doaj   +1 more source

A technical and socioeconomic approach to estimate forest residues as a feedstock for bioenergy in northern Mexico

open access: yesForest Ecosystems, 2019
Background Forest residues can be a feasible alternative for converting energy into fuels, electricity, or heat. Compared to other second-generation bioenergy sources, they do not compete for food, are relatively cheap, abundant in forest-rich areas, and
Guadalupe Carrasco-Diaz   +3 more
doaj   +1 more source

Multiple ETS family transcription factors bind mutant p53 via distinct interaction regions

open access: yesFEBS Letters, EarlyView.
Mutant p53 gain‐of‐function is thought to be mediated by interaction with other transcription factors. We identify multiple ETS transcription factors that can bind mutant p53 and found that this interaction can be promoted by a PXXPP motif. ETS proteins that strongly bound mutant p53 were upregulated in ovarian cancer compared to ETS proteins that ...
Stephanie A. Metcalf   +6 more
wiley   +1 more source

Co-word mapping of Image Retrieval based on Web of Science-Indexed Papers [PDF]

open access: yesمجله علم‌سنجی کاسپین, 2017
Background and aim: Given the special status and wide usage of image retrieval in various fields, the present investigation studied on the research trends and significant factors within the field of image retrieval and drawing the co-word map based on ...
Samira Daniali   +2 more
doaj  

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