Results 231 to 240 of about 36,871 (305)

Rapid development of a transferable Raman model using high‐throughput cell culture for monitoring monoclonal antibody titer

Biotechnology Progress, EarlyView.
Abstract Monoclonal antibody (mAb) titer monitoring is a key capability during process development and optimization, enabling timely decision making and increasing the speed of development. Raman spectroscopy is a prominent process analytical technology (PAT), but resource‐efficient calibration strategies for the development of transferable models are ...
Alexandra Umprecht, Nicholas Uth, Haenah Kim, Oliver Spadiut, Yang Yang   +4 more
wiley   +1 more source

Dual tree complex wavelet transform-based signal denoising method exploiting neighbourhood dependencies and goodness-of-fit test. [PDF]

R Soc Open Sci, 2018
Naveed K, Shaukat B, Ur Rehman N.
europepmc   +1 more source

Investigating the Cytoskeleton of DRGs Using Cryo‐Electron Microscopy and Deep Learning

Cytoskeleton, EarlyView.
ABSTRACT We describe a method for determining the ultrastructural organization of axons and varicosities of cultured dorsal root ganglion (DRG) neurons using cryogenic electron microscopy (cryo‐EM). Cryo‐EM reveals the dimensions, proximity, and overall organization of biological specimens in a near‐native state, avoiding artifacts of fixation and ...
M. Neal Waxham, Mihir Relan, Matthew T. Swulius, Andrea K. H. Stavoe   +3 more
wiley   +1 more source

A new and general approach to signal denoising and eye movement classification based on segmented linear regression. [PDF]

Sci Rep, 2017
Pekkanen J, Lappi O.
europepmc   +1 more source

Engineering and Characterization of a Fluorescent Fission Yeast Arp2/3 Complex for Single Molecule Mechanistic Investigations

Cytoskeleton, EarlyView.
ABSTRACT Arp2/3 complex is a seven‐component protein complex that facilitates the assembly of branched actin filament networks by binding to pre‐existing “mother” actin filaments and initiating the nucleation of new branched “daughter” filaments. Arp2/3 complex must be activated by a nucleation promoting factor such as the WASP/Scar protein family. The
Caitlin A. Anderson, Meghan E. O'Connell, Kaitlin E. Homa, Cristian Suarez, Michael L. James, Kashmeera D. Baboolall, Vilmos Zsolnay, Vladimir Sirotkin, David R. Kovar   +8 more
wiley   +1 more source

Talin1 Adhesion Morphology and Colocalization With Tensin3 Are Largely Unaffected by Polyacrylamide Substrate Stiffness

Cytoskeleton, EarlyView.
ABSTRACT Cells sense the stiffness of their extracellular matrix (ECM) and adapt their behavior accordingly. We investigated how ECM stiffness affects the spatial organization of talin1, a key mechanosensitive focal adhesion protein. Using polyacrylamide (PA) hydrogels with tunable stiffnesses (0.2–188 kPa), we analyzed cell morphology, migration ...
Joanna Hajduk, Patrycja Twardawa, Sylwia Bobis‐Wozowicz, Marta Szewczyk, Zenon Rajfur, Zbigniew Baster   +5 more
wiley   +1 more source

Advances in vital‐sign prediction and early‐warning models for underground coal mine workers integrating environmental factors

Deep Underground Science and Engineering, EarlyView.
This review synthesizes advances in predicting miners' vital signs by integrating environmental monitoring (dust, temperature, and gas) with physiological data. It highlights multi‐source data fusion techniques and early‐warning models for enhanced occupational safety in underground coal mines.
Junji Zhu, Dawei Cui, Yingjie Liu, Yuntao Liang, Yue Zhang   +4 more
wiley   +1 more source

Investigation on tunnel stability considering pipe‐roof support with transparent soil technology

Deep Underground Science and Engineering, EarlyView.
This study integrates transparent soil technology, 3D reconstruction and numerical simulation to captures the full‐field deformation of shield tunnel instability. Results demonstrate that pipe‐roof support enhances stability via the “soil‐arching barrier effect,” effectively restraining failure wedge development by extending stress transfer paths ...
Zhi Jia, Yunpeng Ji, Zhao Lu, Honghua Zhao, Limin Zhang   +4 more
wiley   +1 more source

Inverse sky islands: lowland river valleys drive microbial divergence while high elevations select for convergence in massive mountain ecosystems

Ecography, EarlyView.
Mountain ecosystems are often interpreted through the lens of the ‘sky island' model, where high‐elevation habitats function as isolated archipelagos. However, this model's applicability to massive, topographically complex mountain ranges where highlands are continuous and lowlands are fragmented remains untested.
Yazhou Zhang, J. Aaron Hogan, Jianjun Wang, Wenguang Sun, Yaojun Ye, Xin Liu, Minshu Song, Hang Sun   +7 more
wiley   +1 more source

Nanophotonic Biosensors and the Paradigm of Trans‐Scale Autonomous Bio‐Integrated Diagnostics for Addressing Health Complexities

Electron, EarlyView.
Physics‐driven advances in optical nanobiosensors for rapid, miniaturized, and point‐of‐care diagnostics for next‐generation decentralized and personalized healthcare based on sensor intelligence. ABSTRACT Public health emergencies and the escalating burden of chronic diseases necessitate a paradigm shift from centralized laboratory testing to rapid ...
Vishal Chaudhary, Harsh Sable, Sonu Sonu, Lakshita Phor, Reddicherla Umapathi, Pradeep Bhadola   +5 more
wiley   +1 more source