Results 81 to 90 of about 103,109 (304)

Acidified Blue Ink-staining Procedure for the Observation of Fungal Structures Inside Roots of Two Disparate Plant Lineages

open access: yesBio-Protocol, 2020
Identifying microscopic mycorrhizal fungal structures in roots, i.e., hyphae, vesicles and arbuscules, requires root staining procedures that are often time consuming and involves chemicals known to present health risks from exposure.
Jill kowal, Elena Arrigoni, Sophie Lane
doaj   +1 more source

A Simple Trigonometric Classification of Quartic Roots

open access: yesCoRR
This article provides a simple trigonometric method for determining how many roots of a quartic equation are real and how many are complex, without solving the equation. The approach replaces the quartic's classical discriminant -- a degree-six polynomial in the coefficients -- with an elementary analysis of the function $f(θ) = a\cosθ+ \cos 4θ+ b$ on $
openaire   +3 more sources

Geometric mean Newton’s method for simple and multiple roots

open access: yesApplied Mathematics Letters, 2008
The classical Newton iteration formula to find a root of the equation \(f(x)= 0\) where \(f\) is a sufficiently differentiable real function is \(x_{n+1}= x_n- f(x_n)/f'(x_n)\). Because \[ f(x)= f(x_n)+ \int^x_{x_n} f'(y)\,dy, \] the Newton iteration formula can be interpreted as an approximation of the integral by \((x- x_n)\cdot f'(x_n)\).
Tibor Lukic, Nebojsa M. Ralevic
openaire   +2 more sources

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

open access: yesFEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point   +7 more
wiley   +1 more source

Evaluation of Appropriate Storage Technologies for Shelf-life Improvement of Cassava (Manihot esculenta Crantz) Roots for Marginal Upland Farmers

open access: yesAnnals of Tropical Research, 2014
Fast deterioration of roots after harvest is on of the major challenges in cassava production. This study was conducted to evaluate the effectiveness of simple cassava storage techniques in minimizing postharvest losses. Four storage techniques, namely:
Marcelo A. Quevedo   +2 more
doaj   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

open access: yesFEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

open access: yesFEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn   +4 more
wiley   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

open access: yesFEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei   +3 more
wiley   +1 more source

Protocol for Increasing Carotenoid Levels in the Roots of Citrus Plants

open access: yesBio-Protocol, 2016
Carotenoids in plants play several key functions such as acting as light-harvesters, antioxidants (Lado et al., 2016) or being precursors of strigolactones, abscisic acid, volatiles and other signaling compounds (Arbona et al., 2013).
Matías Manzi   +3 more
doaj   +1 more source

Analysis of Root System Architecture Affected by Swarming Behavior

open access: yesJournal of Horticultural Research, 2020
The root system architecture (RSA) displays complex morphological characteristics because of diverse root growth behaviors. Recent studies have revealed that swarming behavior among roots is particularly important for RSA to adapt to environmental ...
Li Songyang   +3 more
doaj   +1 more source

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