Results 91 to 100 of about 510,826 (300)
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Scriptural Simplicity and Thematic Complexity in L’étranger by Albert Camus [PDF]
UirtusAlbert Camus, in L’étranger, adopts a simple, spare writing style to explore some highly complex themes. The novel is characterized by short, direct sentences, an everyday lexicon and an absence of elaborate figures of speech.
Lakaza Borozi, Piyabalo Bakolou
doaj +1 more source
FEBS Open Bio, EarlyView.This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake +4 more
wiley +1 more source
, 1986 Envelope for a Simplicity pattern to make a Misses' size dress in five sheath and dropped waist styles, including illustrations of the finished pieces and information about sizing and construction on the ...
Simplicity Pattern Co.
core
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
FEBS Open Bio, EarlyView.Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
La fausse simplicité de la prise de parole en classe de langue étrangère
Glottodidactica, 2019 Although the importance of oral production in language learning is recognized, it remains a challenge to convince students to speak. Certainly, this is about daring to take risks, because to speak is to “expose one’s face”.
Sylwia Łuszczyńska
doaj +1 more source
FEBS Open Bio, EarlyView.Cytosolically synthesized chloroplast preproteins are translocated across the outer and inner envelope membranes through translocons called TOC and TIC, respectively. In green algae and plants, the TIC core is composed of essential membrane proteins, Tic12, Tic20, and Tic214.
Mengyi Li, Xueyang Zhao, Masato Nakai
wiley +1 more source
, 1980 Envelope for a Simplicity pattern to make a Misses' topper jacket with bias shawl collar and raglan sleeves, including illustrations of the finished pieces and information about sizing and construction on the ...
Simplicity Pattern Co.
core +1 more source
Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system
FEBS Open Bio, EarlyView.An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková +3 more
wiley +1 more source
, 1985 Simplicity Super Saver Pattern #7105 to make a straight, sleeveless, misses' jumper with a v-neckline and patch pockets, including illustrations of the finished pieces and information about sizing and construction on the ...
Simplicity Pattern Co.
core +1 more source