Synergistic perspectives—How single‐molecule biophysics complement biochemical understanding
In this review, we discuss how ensemble biochemistry and single‐molecule approaches are complementary, outline commonly used single‐molecule techniques, and illustrate their relevance through two representative case studies: chromatin organization by SMC complexes and pathway choice during DNA double‐strand break repair.
Sara De Bragança +2 more
wiley +1 more source
Assessing the COVID-19 Lockdown Impact on Global Air Quality: A Transportation Perspective
To address the COVID-19 pandemic, governments worldwide implemented mandatory restrictions. As an unintended consequence of these responses, significant air pollution reductions have been recorded across the world.
Meina Zheng, Feng Liu, Meichang Wang
doaj +1 more source
Modelling and Simulation of Ternary Liquid-Phase Adsorption onto Activated Carbon
A computational procedure is presented for solving the set of rigid hyperbolic and parabolic partial differential equations describing the simultaneous adsorption of a ternary system in a column packed with adsorbent particles using the non-linear ...
Olaosebikan A. Olafadehan +1 more
doaj +1 more source
Structural and biochemical analysis of a B12 superbinder
BtuG proteins are vitamin B12 scavengers in Bacteroides thetaiotaomicron, a dominant human gut bacterium. We present crystal structures of three BtuG homologs bound to cobalamin and its precursor cobinamide, revealing picomolar binding affinities, among the highest known for any natural protein.
Jose M. Martinez Felices +3 more
wiley +1 more source
Improving agricultural energy inefficiency is essential for achieving sustainable agricultural development and promoting major agricultural countries to achieve carbon peak and carbon neutrality goals. This paper analyzes agricultural energy inefficiency
Biaowen Xu, Xueli Chen
doaj +1 more source
APPLICATION OF SIMULTANEOUS EQUATIONS MODEL TO ESTIMATE PARTICLEBOARD DEMAND AND SUPPLY [PDF]
Dynamic supply and demand equations for particleboard using a three-stage squares simulation (3SLS) were estimated. Empirical data included annual observations over the period of 1976 to 2006.
Anjang Tajdini +4 more
doaj
A simultaneous equation model to determine taper curve.
A simultaneous equation model to determine taper curve for Scots pine is presented. The diameters at relative heights are endogenous variables and height an exogenous variable. Any equations may be substituted by the measured value of diameter.
Kilkki, Pekka +2 more
doaj +1 more source
The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF‐Tu for AMPylation
Fic enzymes mediate diverse post‐translational modifications across all domains of life, including AMPylation. Prokaryotic EF‐Tu can be AMPylated and deAMPylated by the conserved Fic enzyme SoFic. Structural and biochemical approaches were used to characterize the effect of AMPylation on EF‐Tu, SoFic's enzymatic activities, and the enzyme‐target ...
Svenja Runge +6 more
wiley +1 more source
Chiral separation of chloroalkanes with the chromatographic column onboard Martian rovers
Computer image of the Rosalind Franklin Rover of ESA's ExoMars mission. ExoMars is scheduled to land on planet Mars in Oxia Planum in 2029. This area represents an interesting spot to look for biosignatures. Investigations of ExoMars include measurement on molecular chirality. We show that chiral chloroalkanes, that have been identified on Mars, can be
Asma Merzougui +4 more
wiley +1 more source
Membrane composition and thermodynamic identity as boundaries of life for synthetic cell research
What makes a cell a cell? The boundary of a living cell is not just a wall. Read as a Markov blanket, the membrane separates internal from external states, generating identity and non‐equilibrium order. Can this identity be rebuilt from scratch in a synthetic cell?
Caterina Presutti, Bert Poolman
wiley +1 more source

