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Asymmetry of the lipid-bilayer of Sindbis virus
Chemistry and Physics of Lipids, 1976The organization of the lipid bilayer of the enveloped Sindbis virus has been studied. In the model membrane which consists only of two virus specific glycoproteins and host derived lipids the latter were radioactively labelled with 14C-palmitic acid by prelabelling their BHK 21 host cell lipids.
Wilhem Stoffel, Wolfgang Sorgo
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Antibody-mediated activation of sindbis virus
Virology, 1988The biological activity of an anti-Sindbis monoclonal antibody (MCAB 49) has been explored. The antibody recognizes an epitope on the E2 glycoprotein of Sindbis virus and, in the presence of complement (C'), neutralizes virus infectivity. In the absence of C', reaction of the antibody with our laboratory strain of Sindbis, SB, increased the number of ...
Daniel C. Flynn +3 more
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SMALL PLAQUE MUTANTS OF THE SINDBIS VIRUS
1965Abstract : In embryo cell cultures in gel medium infected with the Sindbis virus, two types of plaques appear: large (G) and small (p) plaques. The G plaques appear in 24 hours and grow until they attain a diameter of more than 20mm. The small plaques (p) appear after 48 hours but never attain a diameter exceeding 3mm.
Cl. Hannoun, J. Asso, P. Ardoin
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Plaque variants of Sindbis virus
Archiv f�r die gesamte Virusforschung, 1967Large plaque (G) and small plaque (8) variants were cloned from stock culture of Sindbis virus. Addition of protamine to agar overlay was shown to have little or no effect on the size of plaques produced by G variant, whereas S virus plaque size was significantly increased. This finding suggests that the S variant is more susceptible than the G variant
Yoshiyuki Nagai +6 more
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Crystallization of Sindbis virus and its nucleocapsid
Journal of Molecular Biology, 1992Crystals of Sindbis virus, which contains a lipid-bilayer membrane, have been grown using polyethylene glycol. The space group is R32, a = b = 640 A, c = 1520 A. The crystals are highly mosaic, and recorded diffraction is therefore restricted to spacings of about 30 A.
Stephen C. Harrison +3 more
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Sindbis virus core protein crystals
Journal of Molecular Biology, 1989The core protein of Sindbis virus has been crystallized. Three different crystal forms have been observed. They diffract variously from 2.5 A to 3.5 A resolution.
Ming Luo +7 more
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Location of the Glycoprotein in the Membrane of Sindbis Virus
Nature New Biology, 1971SINDBIS virus, which is transmitted by arthropods, consists of a nucleoprotein core within a lipid-containing envelope. Its components assemble at a cellular membrane and virus particles form by an outfolding of this membrane. Thus, such viruses provide useful systems for studies of the structure and synthesis of membranes.
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Lipid and protein organization in Sindbis virus
Journal of Molecular Biology, 1971Abstract The radial electron density distribution in the Sindbis virus particle has been determined to a resolution of 28 A from measurements of spherically averaged X-ray diffraction. The most striking feature of the density profile is a deep minimum at r = 232A, from which we infer that the lipids of Sindbis virus are organized in a bilayer at ...
J. Jummblatt +3 more
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Glycopeptides of the membrane glycoprotein of Sindbis virus
Journal of Molecular Biology, 1970The membrane of the Sindbis virus (an arbovirus) is structurally simple; it is composed of lipids and a single glycoprotein of molecular weight estimated at 53,000 daltons. Digestion of the membrane glycoprotein with pronase yields a set of glycopeptides (largely polysaccharide) that can be characterized by molecular sieving. From virus grown in chick
Burge, Boyce W., Strauss, James H., Jr.
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