Results 151 to 160 of about 10,820,161 (256)

Gradient-Free Isolation of Murine Pancreatic Islets for Single-Cell RNA Sequencing. [PDF]

Methods Protoc
Ruchko ES, Starinnov ZR, Sabirov MS, Chernysheva MB, Lee AA, Gasanov VAO, Vasiliev AV.   +6 more
europepmc   +1 more source

Small RNA pathways in mammalian oocytes

FEBS Open Bio, EarlyView.
Three distinct small RNA pathways operate in mammalian oocytes: RNAi interference (RNAi), the microRNA (miRNA) pathway, and the PIWI‐associated RNA (piRNA) pathway. These pathways use small RNAs to guide sequence‐specific repression and contribute to oocyte biology by targeting genes and mobile elements or appear insignificant since different ...
Petr Svoboda, Josef Pasulka
wiley   +1 more source

Single-cell RNA-sequencing of RVFV infection

On the RNA level, viral infections are characterized by perturbations in the host cell transcriptome as well as the development of viral genetic information.
Wyler, E.
core   +1 more source

Transformers for single-cell RNA sequencing: a survey. [PDF]

Brief Bioinform
Hu T, Wei Z.
europepmc   +1 more source

Transcripts enriched in codons that trigger P‐site tRNA‐mediated mRNA decay possess stable mRNA

FEBS Open Bio, EarlyView.
PTMD codons were first described by Mendel et al. as mediators of an mRNA decay pathway dependent on the human protein CNOT3, homologous to yeast Not5. Our findings confirm that PTMD codons destabilize transcripts; however, unlike in yeast, the human pathway specifically targets and slightly destabilizes primarily stable mRNAs.
Rodolfo Lopes Carneiro, Fernando Lucas Palhano   +1 more
wiley   +1 more source

Accurate quantification of spliced and unspliced transcripts for single-cell RNA sequencing with tidesurf. [PDF]

PLoS One
Schleicher JT, Klingler D, Claassen M.
europepmc   +1 more source

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

FEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya, Masatake Osawa, Hidefumi Fukumitsu   +2 more
wiley   +1 more source

Single-cell RNA sequencing data processing using cloud-based serverless computing. [PDF]

GigaByte
Hung LH, Nasam N, Biju C, Lloyd W, Yeung KY.   +4 more
europepmc   +1 more source

Evaluating the involvement of autolysosomes in the nuclear translocation of fluorescent proteins

FEBS Open Bio, EarlyView.
Endogenously expressed fluorescent proteins can be degraded by autophagy and transported to cell nuclei via the nuclear pore complex. But in some cell lines, for example, HeLa cells which are positive for immunoreactivity of a receptor ligand, such as UCN I, in cell nuclei, fusion of autolysosome with the nuclear envelope is involved in the nuclear ...
Keiichi Ikeda
wiley   +1 more source

Single-Cell RNA Sequencing Reveals Immune Dysregulation and Candidate Drug Targets in Endometriosis. [PDF]

Cells
Rezk-Füreder M, Kazemi M, Gyunesh AA, Bryant SD, Oppelt P, Hamzic-Jahic E, Reisinger D, Kapper C, Demmel D, Lackner A, Lang K, Arbeithuber B.   +11 more
europepmc   +1 more source