Results 221 to 230 of about 1,198,587 (324)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Metformin promotes mitochondrial integrity through AMPK‐signaling in Leber's hereditary optic neuropathy

FEBS Open Bio, EarlyView.
Metformin mediates mitochondrial quality control in Leber's hereditary optic neuropathy (LHON) fibroblasts carrying mtDNA mutations. At therapeutic levels, metformin activates AMPK signaling to restore mitochondrial dynamics by promoting fusion and restraining fission, while preserving mitochondrial mass, enhancing autophagy/mitophagy and biogenesis ...
Chatnapa Panusatid, Rapasviranda Soiyangsuk, Maneeluck Tanadjindarat, Chayanon Peerapittayamongkol   +3 more
wiley   +1 more source

Single-cell RNA sequencing identifies ZBP1-dependent mechanisms in OSCC progression. [PDF]

Cell Death Dis
Lin X, Wang C, Li C, Wu J, Zhang P, Zhang C, Chen H, Xu F, Zhang S, Luo C, Tang C.   +10 more
europepmc   +1 more source

Comparative Analysis of Single-Cell RNA Sequencing Methods

bioRxiv, 2016
C. Ziegenhain, B. Vieth, Swati Parekh, B. Reinius, Martha Smets, H. Leonhardt, Ines Hellmann, W. Enard   +7 more
semanticscholar   +1 more source

FGFR Like1 drives esophageal cancer progression via EMT, PI3K/Akt, and notch signalling: insights from clinical data and next‐generation sequencing analysis

FEBS Open Bio, EarlyView.
Clinical analysis reveals significant dysregulation of FGFRL1 in esophageal cancer (EC) patients. RNAi‐coupled next‐generation sequencing (NGS) and in vitro study reveal FGFRL1‐mediated EC progression via EMT, PI3K/Akt, and Notch pathways. Functional assays confirm its role in tumor growth, migration, and invasion.
Aprajita Srivastava, Anoop Saraya, Deepak Gunjan, Rinu Sharma   +3 more
wiley   +1 more source

A protocol for high-quality single-cell RNA sequencing with cell surface protein quantification. [PDF]

Blood Sci
Han S, Liu S, Chen C.
europepmc   +1 more source

MULTI-seq: sample multiplexing for single-cell RNA sequencing using lipid-tagged indices

Nature Methods, 2019
Christopher S. McGinnis, David M. Patterson, Juliane Winkler, Daniel N. Conrad, Marco Y. Hein, Vasudha Srivastava, Jennifer L Hu, Lyndsay M. Murrow, J. Weissman, Z. Werb, E. Chow, Zev J. Gartner   +11 more
semanticscholar   +1 more source

KLK7 overexpression promotes an aggressive phenotype and facilitates peritoneal dissemination in colorectal cancer cells

FEBS Open Bio, EarlyView.
KLK7, a tissue kallikrein‐related peptidase, is elevated in advanced colorectal cancer and associated with shorter survival. High KLK7 levels in ascites correlate with peritoneal metastasis. In mice, KLK7 overexpression increases metastasis. In vitro, KLK7 enhances cancer cell proliferation, migration, adhesion, and spheroid formation, driving ...
Yosr Z. Haffani, Tobias Dreyer, Meriem Naim, Rea Lo Dico, Natalia A. Ignatenko, Viktor Magdolen, Dalila Darmoul   +6 more
wiley   +1 more source

Single-Cell RNA Sequencing of Thyroid Tissues Reveals Pathogenesis of Graves' Disease. [PDF]

Adv Sci (Weinh)
Zhou X, Cong J, Peng R, Yang D, Dong C, Xie J, Yan J, Kuang J, Li F, Yeap LS, Xie X, Yin H, Han R, Shen L, Zhou Y, Ning G, Wang S, Wang W, Ye L.   +18 more
europepmc   +1 more source

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source