Results 41 to 50 of about 63,316 (241)
Communications Biology, 2023 The spatial organization and intracellular dynamics of the stressosome proteins in L. monocytogenesare revealed using photo-activated localization microscopy combined with single-particle tracking and single-molecule displacement mapping.
Buu Minh Tran +9 more
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Tracking Single Particles and Elongated Filaments with Nanometer Precision [PDF]
Biophysical Journal, 2011 Recent developments in image processing have greatly advanced our understanding of biomolecular processes in vitro and in vivo. In particular, using Gaussian models to fit the intensity profiles of nanometer-sized objects have enabled their two-dimensional localization with a precision in the one-nanometer range.
Ruhnow +3 more
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Optimal Diffusion Coefficient Estimation in Single-Particle Tracking [PDF]
Biophysical Journal, 2012 Single-particle tracking is increasingly used to extract quantitative parameters on single molecules and their environment, while advances in spatial and temporal resolution of tracking techniques inspire new questions and avenues of investigation.
Berglund, Andrew J., Michalet, Xavier
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eLife, 2015 Myosin 5a is a dual-headed molecular motor that transports cargo along actin filaments. By following the motion of individual heads with interferometric scattering microscopy at nm spatial and ms temporal precision we found that the detached head ...
Joanna Andrecka +8 more
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FEBS Letters, EarlyView.Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho +3 more
wiley +1 more source
eLife, 2019 We present the development and in vivo application of a feedback-based tracking microscope to follow individual mitochondria in sensory neurons of zebrafish larvae with nanometer precision and millisecond temporal resolution.
Fabian Wehnekamp +4 more
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Multi-color quantum dot tracking using a high-speed hyperspectral line-scanning microscope. [PDF]
PLoS ONE, 2013 Many cellular signaling processes are initiated by dimerization or oligomerization of membrane proteins. However, since the spatial scale of these interactions is below the diffraction limit of the light microscope, the dynamics of these interactions ...
Patrick J Cutler +5 more
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Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation
FEBS Letters, EarlyView.Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe +3 more
wiley +1 more source
Biomedical Journal, 2022 Time-lapse microscopy images generated by biological experiments have been widely used for observing target activities, such as the motion trajectories and survival states.
Hui-Jun Cheng +3 more
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FEBS Letters, EarlyView.Plasma membranes contain dynamic nanoscale domains that organize lipids and receptors. Because viruses operate at similar scales, this architecture shapes early infection steps, including attachment, receptor engagement, and entry. Using influenza A virus and HIV‐1 as examples, we highlight how receptor nanoclusters, multivalent glycan interactions ...
Jan Schlegel, Christian Sieben
wiley +1 more source